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False-Positive Reactions in the Immunoprecipitation Test for Meat Identification
I R Tizard1, N A Fish1, F Caoili1
1Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Abstract:
During the course of experiments into the specificity of the ring precipitation test it was found that a commercial anti-porcine serum reacted with bovine plasma. When this phenomenon was investigated it was found to be non-immunological in nature and that plasma obtained from blood treated with calcium chelating agents gave a positive reaction with normal serum. The species of origin of the reactants appeared to have no effect on the reaction. Further analysis indicated that a similar false-positive reaction could be obtained using sodium ascorbate either as an anticoagulant or as an additive to ground beef. Preliminary studies were conducted on the mechanism of this reaction.
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