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Usefulness of apolipoprotein B-depleted serum in cholesterol efflux capacity assays using immobilized liposome-bound

Yuna Horiuchi1, Ryunosuke Ohkawa2, Shao-Jui Lai3

  • 1Analytical Laboratory Chemistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.

Bioscience Reports
|March 15, 2019
PubMed

Insights

Cholesterol efflux capacity (CEC) measured using apolipoprotein B-depleted serum (BDS) reflects both high-density lipoprotein (HDL) function and its concentration. This method is a reliable alternative for assessing HDL

Area of Science:

  • Cardiovascular Science
  • Lipid Metabolism
  • Biochemistry

Background:

  • High-density lipoprotein (HDL) plays a key role in preventing atherosclerosis via cholesterol efflux capacity (CEC).
  • Apolipoprotein B-depleted serum (BDS) is increasingly used as a substitute for isolated HDL in CEC assays.
  • The reliability of BDS as a cholesterol acceptor (CA) is debated.

Purpose of the Study:

  • To evaluate the suitability of BDS as a cholesterol acceptor for measuring CEC.
  • To assess whether BDS-derived CEC (BDS-CEC) is influenced by serum contaminants or HDL concentration.

Main Methods:

  • Calculated whole-cholesterol efflux capacity (whole-CEC) by integrating CEC with HDL-cholesterol (HDL-C) levels.
  • Assessed BDS-CEC deviations against serum protein, LDL, and triglyceride levels to identify systematic errors.
  • Tested reconstituted serum samples with varying HDL-C and serum protein levels to evaluate protein effects.

Main Results:

  • A significant correlation (r = 0.633) was found between BDS-CEC and HDL-isolated CEC.
  • Deviations in BDS-CEC were not correlated with serum protein, LDL, or triglyceride levels, ruling out systematic errors.
  • BDS-CEC remained stable across different combinations of serum proteins and HDL-C levels.

Conclusions:

  • BDS-CEC is a valid measure that reflects both HDL function and its concentration in serum.
  • BDS is a suitable cholesterol acceptor for CEC measurements, offering a potentially more practical alternative to isolated HDL.

Keywords:
apolipoprotein B-depleted serumcardiovascular diseasecholesterol efflux capacityhigh-density lipoprotein

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