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Related Experiment Video

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Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

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ELIPatch, a thumbnail-size patch with immunospot array for multiplexed protein detection from human skin surface.

Dong Yoon Oh, Hunjong Na, Seo Woo Song

¹QuantaMatrix, Inc., Seoul 03122, South Korea.

Biomicrofluidics

|March 15, 2019

Summary

This study introduces a small skin patch for detecting multiple protein biomarkers, offering a convenient alternative to bulky methods for diagnosing skin conditions.

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Area of Science:

Biomedical Engineering
Biomarker Discovery
Dermatology

Background:

Secreted skin proteins show promise as biomarkers for skin conditions.
Current detection methods are often inconvenient and bulky.

Purpose of the Study:

To develop a compact, user-friendly device for multiplexed skin protein biomarker detection.
To enable high-spatial-resolution analysis of skin biomarkers.

Main Methods:

Development of a thumbnail-sized patch with an array of antibody-functionalized microdisks.
Utilizing self-assembly for 2D array formation of microdisks on adhesive tape.
Implementation of an Enzyme-Linked Immunospot array on a Patch (ELISPOT-on-Patch) for multiplexed immunoassay.

Main Results:

The ELISPOT-on-Patch demonstrated sufficient sensitivity for detecting IL-1α, IL1RA, IL-17A, IFN-γ, and TNF-α.
IL-6 and IL-1β detection was variable and not consistently achievable.
Successful quantification of cytokines from different skin regions and volunteers was achieved.

Conclusions:

The developed patch offers a sensitive and spatially resolved method for skin biomarker analysis.
This technology presents a promising, portable alternative for non-invasive skin condition diagnostics.
Further optimization may be needed for detecting lower-abundance cytokines like IL-6 and IL-1β.