Related Experiment Video
Updated: Jan 27, 2026

Extraction of High Molecular Weight DNA from Microbial Mats
Published on: July 7, 2011
Bacterial communities of the microbial mats of Chokrak sulfide springs
Ekaterina I Burganskaya1, Irina A Bryantseva2, Maria S Krutkina2
1Research Center of Biotechnology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7 k. 2, 117312, Moscow, Russia. kateburganskaya@gmail.com.
Abstract:
This is the comparative investigation of the composition of phototrophic microbial mats developing in sulfide-rich saline Chokrak springs with outflow at the shore of the hypersaline lake Chokrak by means of next-generation sequencing. The springs are characterized by low temperature (~ 15 °C), near-neutral pH (6.7-8.5), and high-sulfide content. In the species composition the benthic microbial communities of Chokrak springs are similar to microbial mats of marine supralittoral and lagoons. Our results showed that salinity limitation had a significant effect on the species composition of benthic microbial communities developing at the outflow of the Chokrak springs. Predominant oxygenic phototrophs belonged to the genera Phormidium, Lyngbya, Leptolyngbya, Geitlerinema, and Arthrospira. Anoxygenic phototrophic bacteria were represented by halophilic green sulfur bacteria Prosthecochloris spp., halotolerant Chlorobaculum sp., as well as marine and extremely halophilic purple bacteria Roseospira, Rhodovibrio, and Halochromatium. Monoculture of a new species of halotolerant anoxygenic filamentous phototrophic bacteria was isolated.
Related Concept Videos
Gene Regulation in Microbial Communities: Quorum Sensing
What are Populations and Communities?
Frequency of Spring-Mass System
Consider a block on a spring on a frictionless surface. There...
Preparation and Reactions of Sulfides
Bacterial Signaling
Structure and Nomenclature of Thiols and Sulfides

