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SIRT1 promotes GLUT1 expression and bladder cancer progression via regulation of glucose uptake

Jia Chen1, Lin Cao2, Zhiqiu Li1

  • 1Department of Urology Surgery, Hunan People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, China.

Human Cell
|March 15, 2019
PubMed

Insights

Sirtuin 1 (SIRT1) and glucose transporter 1 (GLUT1) are upregulated in bladder cancer. The SIRT1/GLUT1 pathway drives tumor growth by increasing glucose metabolism and cell proliferation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Metabolic Research

Background:

  • Bladder cancer (BC) is a prevalent malignancy characterized by metabolic reprogramming.
  • Sirtuin 1 (SIRT1), a deacetylase, regulates metabolic pathways.
  • The role of SIRT1 in bladder cancer's metabolic phenotype, particularly its interaction with glucose transporter 1 (GLUT1), is not well understood.

Purpose of the Study:

  • To investigate the functional interaction between SIRT1 and GLUT1 in bladder cancer.
  • To elucidate the mechanism by which SIRT1 influences GLUT1 expression and metabolic activity in BC cells.
  • To explore the therapeutic potential of targeting the SIRT1/GLUT1 axis in bladder cancer.

Main Methods:

  • Analysis of SIRT1 and GLUT1 expression in BC tissues and cell lines.
  • Cell proliferation and glucose uptake assays.
  • Overexpression and inhibition studies using SIRT1 and GLUT1.
  • Assessment of GLUT1 transcriptional activity.

Main Results:

  • SIRT1 and GLUT1 expression were elevated and positively correlated in BC tissues and cells.
  • SIRT1 and GLUT1 overexpression independently enhanced BC cell proliferation and glucose uptake.
  • SIRT1 inhibition reduced proliferation and glucose uptake, effects partially rescued by GLUT1 overexpression.
  • SIRT1 overexpression significantly increased GLUT1 transcription and expression.

Conclusions:

  • The SIRT1/GLUT1 axis is a key driver of bladder cancer progression.
  • SIRT1 promotes bladder cancer cell proliferation and glycolysis by upregulating GLUT1 transcription and expression.
  • Targeting the SIRT1/GLUT1 pathway represents a potential therapeutic strategy for bladder cancer.
Keywords:
Bladder cancerCell proliferationGLUT1GlucoseSIRT1

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