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Updated: Jan 27, 2026

Isolation and Characterization of Cyanobacterial Extracellular Vesicles
Published on: February 3, 2022
Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi
Izadora Volpato Rossi1,2, Bruno Gavinho1,2, Marcel Ivan Ramirez3,4
1Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Abstract:
Extracellular vesicles (EVs) are heterogeneous membrane-surrounded structures that participate in cellular communications, which comprise exosomes and microvesicles. These vesicles have different biogenesis, and their physiological and pathological roles in chronic and infectious diseases are under constant investigation. In Chagas disease, Trypanosoma cruzi EVs have been described using different approaches. The isolation of T. cruzi-derived EVs has been done mainly using the differential centrifugation technique, and different strategies have been employed for characterization of them. Here, we describe the method to isolate EVs by differential centrifugation and a detection protocol for EVs in T. cruzi-host cell interaction to allow further investigations about this parasite.
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