Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Surface Membrane Barriers01:18

Surface Membrane Barriers

2.8K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
2.8K
Urea Cycle01:23

Urea Cycle

50.1K
The urea cycle describes how liver cells convert ammonia to urea. Ammonia is a toxic waste product of protein catabolism. Land animals must convert ammonia into the less toxic urea which can be safely eliminated by the kidneys through urine. Marine animals excrete ammonia directly, and the surrounding water dilutes the ammonia to safe levels.
50.1K
Cell-surface Signaling01:21

Cell-surface Signaling

54.2K
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
54.2K
Reconstruction of Signal using Interpolation01:10

Reconstruction of Signal using Interpolation

719
Signal processing techniques are essential for accurately converting continuous signals to digital formats and vice versa. When a continuous signal is sampled with a period T, the resulting sampled signal exhibits replicas of the original spectrum in the frequency domain, spaced at intervals equal to the sampling frequency. To handle this sampled signal, a zero-order hold method can be applied, which creates a piecewise constant signal by retaining each sample's value until the next...
719
Surface Tension and Surface Energy01:16

Surface Tension and Surface Energy

3.2K
When a paint brush is immersed in water, the bristles wave freely inside the water. When it is taken out, the bristles stick together. The reason behind this effect is surface tension.
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...
3.2K
The Resting Membrane Potential01:21

The Resting Membrane Potential

142.1K
Overview
142.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Noncontact Esthesiometer: Large Cohort Study on Agreement with Contact Method and Correlations with Corneal Nerves and Ocular Surface.

Ophthalmology science·2026
Same author

Editorial: Ocular surface disorders- an insight.

Frontiers in ophthalmology·2026
Same author

Spark plasma sintered titania-graphene oxide: A toothless keratoprosthesis for end-stage corneal blindness.

Biomaterials·2026
Same author

Multimodal Imaging of the Corneal Endothelial Transition Zone Reveals Progenitor Cell Population.

Cells·2025
Same author

Deep learning-assisted widefield endothelial imaging in Descemet membrane endothelial keratoplasty.

Frontiers in medicine·2025
Same author

Cell Counting and Cell Cycle Analysis of Simple Non-Cultured Endothelial Cell Injection (SNEC-I) Therapy: Characterization for Clinical Translation.

Cells·2025

Related Experiment Video

Updated: Jan 27, 2026

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
10:00

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

8.3K

Urea-De-Epithelialized Human Amniotic Membrane for Ocular Surface Reconstruction.

Francisco Bandeira1,2,3, Gary Hin-Fai Yam1,4, Matthias Fuest1,5

  • 1Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore.

Stem Cells Translational Medicine
|March 15, 2019
PubMed
Summary

A novel, cost-effective method using urea-treated human amniotic membrane (uHAM) successfully created tissue-engineered conjunctival grafts. These grafts effectively reconstructed ocular surfaces in patients with severe conjunctival disorders.

Keywords:
Cell cultureConjunctivaDe-epithelializationHuman amniotic membraneOcular surface reconstructionTissue engineering

More Related Videos

Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
04:21

Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature

Published on: October 14, 2022

1.8K
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

9.8K

Related Experiment Videos

Last Updated: Jan 27, 2026

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
10:00

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

8.3K
Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
04:21

Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature

Published on: October 14, 2022

1.8K
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

9.8K

Area of Science:

  • Ophthalmology
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Conjunctival diseases necessitate surgical intervention, often involving large tissue excisions.
  • Current tissue engineering methods for conjunctival defects using human amniotic membrane (HAM) face challenges with time-consuming, expensive, or damaging epithelial removal protocols.
  • Previous research introduced an efficient ice-cold urea method for de-epithelializing HAM (uHAM).

Observation:

  • Two patients with a giant conjunctival nevus and a large symblepharon underwent ocular surface reconstruction.
  • Autologous conjunctival epithelial cells were cultured on uHAM scaffolds.
  • Tissue-engineered constructs were transplanted immediately after lesion removal.

Findings:

  • Successful reconstruction of the ocular surface was achieved in both patients using the uHAM scaffold.
  • No recurrence of lesions or epithelial defects were observed during follow-up periods of up to 7 and 19 months.
  • The uHAM method proved efficient and inexpensive for preparing the basement membrane scaffold.

Implications:

  • This study demonstrates the translational potential of the uHAM method for treating ocular surface disorders.
  • The findings support the use of cell-based tissue-engineered treatments for conjunctival reconstruction.
  • The efficient and economical uHAM preparation offers a promising alternative for clinical applications.