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Detection and molecular characterisation of bovine Enterovirus in Brazil: four decades since the first report
M Candido1, S R Almeida-Queiroz1, M G Buzinaro2
1Department of Veterinary Medicine,Faculty of Animal Science and Food Engineering,University of São Paulo (FZEA/USP),Avenue Duque de Caxias Norte,225, Jardim Elite,Pirassununga,São Paulo 13635-900,Brazil.
Abstract:
It is suggested that bovine enteroviruses (BEV) are involved in the aetiology of enteric infections, respiratory disease, reproductive disorders and infertility. In this study, bovine faecal samples collected in different Brazilian states were subjected to RNA extraction, reverse transcription-polymerase chain reaction analysis and partial sequencing of the 5'-terminal portion of BEV. One hundred and three samples were tested with an overall positivity of 14.5%. Phylogenetic analysis clustered these BEV Brazilian samples into the Enterovirus F clade. Our results bring an important update of the virus presence in Brazil and contribute to a better understanding of the distribution and characterisation of BEV in cattle.
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