Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.8K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.8K
CRISPR01:59

CRISPR

57.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.8K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.0K
RNA Editing02:23

RNA Editing

9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
Genomics02:02

Genomics

40.3K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.3K
Sympathetic Pathways: Collateral Ganglia and Adrenal Medulla01:27

Sympathetic Pathways: Collateral Ganglia and Adrenal Medulla

2.9K
The sympathetic pathways of the collateral ganglia and adrenal medulla serve unique but interconnected roles in the sympathetic response.
Collateral Ganglia
Sympathetic preganglionic axons reach the collateral ganglia along the route of splanchnic nerves. These nerves bypass the sympathetic trunk and communicate with sympathetic postganglionic neurons housed in the prevertebral ganglia. These ganglia supply the organs of the abdominopelvic cavity.
The greater splanchnic nerve, formed by the...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Large distant deletion disrupts CDKN2A enhancer and predisposes to melanoma.

medRxiv : the preprint server for health sciences·2026
Same author

GDF15 Reprograms the Microenvironment to Drive Liver Metastasis of Uveal Melanoma.

Cancer research·2026
Same author

Novel fusion gene THBS1::ERBB2 drives a subset of superficial acral fibromyxomas.

The British journal of dermatology·2026
Same author

Targeting noncanonical nuclear factor kappa B signalling in CYLD cutaneous syndrome by selective inhibition of IκB kinase alpha.

The British journal of dermatology·2026
Same author

The oncogenome of the domestic cat.

Science (New York, N.Y.)·2026
Same author

Ancestry and somatic profile indicate acral melanoma origin and prognosis.

Nature·2026
Same journal

Genetic survey of biomarkers at early and mid-pregnancy identifies pregnancy-specialized immune regulation.

PLoS genetics·2026
Same journal

Argonaute proteins orchestrate Meiotic Sex Chromosome Inactivation and timing of the spermatogenic transcriptional program.

PLoS genetics·2026
Same journal

Genome wide association study meta-analysis of neuropathologic lesions of Alzheimer's disease and related dementias in a multi-site autopsy cohort.

PLoS genetics·2026
Same journal

Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.

PLoS genetics·2026
Same journal

Multiple instance fine-mapping: Predicting causal regulatory variants with a deep sequence model.

PLoS genetics·2026
Same journal

Nuclear ubiquitin-conjugating enzyme TrUbc4 and F-box protein TrFwd1-mediated modification of Cre1 in Trichoderma reesei establishes a regulatory mechanism for carbon catabolite repression.

PLoS genetics·2026
See all related articles

Related Experiment Video

Updated: Jan 27, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

23.2K

Collateral damage and CRISPR genome editing.

Mark Thomas1, Gaetan Burgio2, David J Adams1

  • 1Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom.

Plos Genetics
|March 15, 2019
PubMed
Summary
This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR-Cas9) gene editing can cause unintended deletions at target sites. While both studies found deletions, they reported different conclusions regarding accuracy and safety for therapeutic applications.

More Related Videos

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.0K
Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
08:00

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Published on: October 27, 2019

10.4K

Related Experiment Videos

Last Updated: Jan 27, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

23.2K
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.0K
Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
08:00

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Published on: October 27, 2019

10.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • CRISPR-Cas systems offer versatile gene editing but raise accuracy concerns.
  • Cas endonuclease effectors are crucial for precise gene modification.
  • Therapeutic applications require high accuracy in gene editing.

Purpose of the Study:

  • To investigate the accuracy of CRISPR-Cas9 gene editing.
  • To define the landscape of off-target mutations induced by CRISPR-Cas9.
  • To detail on-target, potentially deleterious deletions caused by CRISPR-Cas9.

Main Methods:

  • Comparative analysis of two independent studies on CRISPR-Cas9 editing accuracy.
  • Characterization of off-target mutations.
  • Identification and analysis of on-target deletions.

Main Results:

  • Both studies identified large on-target deletions induced by CRISPR-Cas9.
  • One study defined the landscape of off-target mutations.
  • The studies reached different conclusions despite similar findings on deletions.

Conclusions:

  • CRISPR-Cas9 gene editing can result in significant on-target deletions.
  • Further research is needed to reconcile differing conclusions on CRISPR-Cas9 accuracy.
  • Ensuring precision is critical for the therapeutic use of CRISPR-Cas9 technology.