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Iridium oxide (IV) nanoparticle-based lateral flow immunoassay.

Daniel Quesada-González1, Amadeo Sena-Torralba1, Wiyogo Prio Wicaksono2

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Researchers introduce iridium oxide nanoparticles for lateral flow biosensors, enabling sensitive detection of human immunoglobulin. This novel approach leverages the nanoparticles

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Iridium oxide nanoparticlesLateral flow immunoassayMobile phone sensingPaper-based biosensor

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Lateral flow biosensors offer rapid, selective, and low-cost analyte detection.
  • Nanomaterials enhance lateral flow biosensor performance through optical or electrical detection.
  • Novel nanomaterials are sought to improve sensing capabilities.

Purpose of the Study:

  • To introduce iridium oxide (IrO) nanoparticles as novel labels for lateral flow assays.
  • To demonstrate the use of IrO nanoparticles for detecting human immunoglobulin as a model protein.

Main Methods:

  • Conjugation of iridium oxide nanoparticles with biomarkers.
  • Development of a lateral flow assay utilizing IrO nanoparticle labels.
  • Visual detection based on the colorimetric properties of IrO nanoparticles.

Main Results:

  • Iridium oxide nanoparticles were successfully prepared and conjugated with biomarkers.
  • The dark blue color of IrO nanoparticles provided high contrast on white lateral flow strips.
  • The proposed method demonstrated effective detection of human immunoglobulin.

Conclusions:

  • Iridium oxide nanoparticles are suitable and advantageous labels for lateral flow assays.
  • This novel application offers a high-contrast, cost-effective sensing strategy.
  • The use of IrO nanoparticles expands the toolkit for developing advanced biosensors.