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Updated: Jan 27, 2026

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
Published on: January 7, 2019
Effect of light conditions on mixotrophic cultivation of green microalgae
Anil Kumar Patel1, Jae Min Joun1, Min Eui Hong1
1Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seoungbuk-gu, Seoul 02841, Republic of Korea.
Abstract:
Current research aimed to increase mixotrophic biomass from various organic carbon sources by exploring best light conditions. Three substrates glucose, acetic acid and glycerol were studied for their effects on mixotrophic microalgae cultivation under four light conditions. Light irradiance exhibited variability in growth response and photosynthetic efficiency based on type of substrates used in mixotrophic growth. Each substrate showed variability in light requirements for their effective assimilations. From growth responses, glucose and acetic acid respectively exhibited heterotrophic and mixotrophic (better growth in light) natures. Continuous light-deficient condition was adequate for effective mixotrophic growth as well as energy saving for glucose. However, light-sufficient condition required for effective acetic acid supported mixotrophic growth. Mixotrophic benefits from glycerol and its uptake by Chlorella protothecoides was negligible in all light conditions. Investigation of heterotrophic biomass contribution by various substrates in overall mixotrophic yield, glucose offered maximum approx. 43% contribution.
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