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Long-Term Exposure to Oroxylin A Inhibits Metastasis by Suppressing CCL2 in Oral Squamous Cell Carcinoma Cells

Wei-Ting Ku1, Jiun-Jia Tung2,3, Tony Jer-Fu Lee4,5

  • 1Master Program of Pharmacology and Toxicology, Department of Medicine, School of Medicine, Tzu Chi University, Hualien 97004, Taiwan. 106721104@gms.tcu.edu.tw.

Cancers
|March 16, 2019
PubMed

Insights

Oroxylin A (Oro-A) significantly inhibits oral squamous cell carcinoma (OSCC) migration and metastasis. Long-term Oro-A treatment suppresses cancer cell migration by downregulating CCL2 signaling pathways.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Oroxylin A (Oro-A), a flavonoid from Scutellaria radix, shows anti-migration effects in cancer.
  • Oral squamous cell carcinoma (OSCC) is a significant global health concern with migratory potential.

Purpose of the Study:

  • To investigate the anti-migration effects of Oro-A on OSCC cells.
  • To elucidate the molecular mechanisms behind Oro-A's anti-migratory action in OSCC.

Main Methods:

  • Wound-healing assays for cell migration assessment.
  • cDNA microarray and Ingenuity software for gene expression analysis.
  • Quantitative real-time PCR (Q-PCR), Western blotting, and ELISA for validating gene and protein expression (CCL2, p-ERK1/2, NFκB, MMP2, MMP9).

Main Results:

  • Short-term and long-term Oro-A exposure significantly inhibited OSCC cell migration.
  • Long-term Oro-A treatment led to significant downregulation of migration-related genes, including CCL2.
  • Oro-A suppressed CCL2 signaling pathway components and downstream targets, inhibiting in vivo metastasis.

Conclusions:

  • Long-term Oroxylin A treatment effectively inhibits OSCC cell migration and metastasis.
  • The anti-metastatic effect of Oro-A is mediated through the downregulation of the CCL2 signaling pathway.
  • Oro-A demonstrates potential as a therapeutic agent for OSCC treatment.
Keywords:
CCL2metastasisoral squamous cell carcinomaoroxylin A

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