Related Experiment Video

Updated: Jan 27, 2026

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

12.0K

Transcriptome profiling analysis reveals that CXCL2 is involved in anlotinib resistance in human lung cancer cells

Jun Lu1, Wei Xu2, Jie Qian1

  • 1Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, 241 Huaihai West Rd, Shanghai, 200030, China.

BMC Medical Genomics
|March 16, 2019
PubMed
Abstract

Insights

Anlotinib resistance in non-small cell lung cancer (NSCLC) involves the CXCL2 gene. This study identifies CXCL2 as a key factor contributing to anlotinib resistance, offering insights into treatment mechanisms.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Anlotinib demonstrates anti-tumor efficacy in non-small cell lung cancer (NSCLC) as a third-line treatment.
  • Anlotinib resistance is a clinical challenge with an unclear underlying mechanism.

Purpose of the Study:

  • To investigate the molecular mechanisms of anlotinib resistance in NSCLC.
  • To identify genes associated with anlotinib resistance and their functional roles.

Main Methods:

  • Established an in vitro anlotinib-resistant non-small cell lung cancer (NSCLC) cell line (NCI-H1975).
  • Performed transcriptome profiling to identify differentially expressed genes between sensitive and resistant cells.
  • Conducted functional assays to validate the role of CXCL2 in anlotinib resistance.

Main Results:

  • Anlotinib inhibited proliferation in sensitive cells but showed reduced efficacy in resistant cells.
  • Transcriptome analysis revealed 769 anlotinib-responsive genes and identified 127 genes associated with resistance.
  • CXCL2 gene expression was found to be significantly correlated with and contribute to anlotinib resistance.

Conclusions:

  • CXCL2 plays a crucial role in the development of anlotinib resistance in non-small cell lung cancer (NSCLC).
  • Understanding the involvement of CXCL2 provides potential therapeutic targets for overcoming anlotinib resistance.
Keywords:
AnlotinibCXCL2Drug resistanceNon-Small Cell Lung CancerTranscriptome

More Related Videos

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level09:45

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

3.3K
RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

22.4K

Related Concept Videos

Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.7K
Cancer Survival Analysis01:21

Cancer Survival Analysis

Cancer survival analysis focuses on quantifying and interpreting the time from a key starting point, such as diagnosis or the initiation of treatment, to a specific endpoint, such as remission or death. This analysis provides critical insights into treatment effectiveness and factors that influence patient outcomes, helping to shape clinical decisions and guide prognostic evaluations. A cornerstone of oncology research, survival analysis tackles the challenges of skewed, non-normally...
665
Lung Capacity01:47

Lung Capacity

The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures.
56.2K
Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.5K
Resistance01:19

Resistance

When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
5.9K
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
1.4K