Related Experiment Video

Updated: Jan 27, 2026

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents
09:28

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

Published on: September 16, 2020

9.3K

Pharmacological HIF inhibition prevents retinal neovascularization with improved visual function in a murine

Yukihiro Miwa1, Yusuke Hoshino2, Chiho Shoda3

  • 1Laboratory of Photobiology, Keio University School of Medicine, Tokyo, Japan; Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.

Neurochemistry International
|March 16, 2019
PubMed

Insights

Hypoxia-inducible factor (HIF) inhibition effectively treats pathological retinal neovascularization without harming physiological levels. This approach may offer a new therapy for blindness-causing retinal diseases and associated vision loss.

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Pharmacology

Background:

  • Neovascular retinal diseases are a primary cause of blindness globally.
  • Anti-vascular endothelial growth factor (VEGF) therapies are effective but can cause chorioretinal atrophy.
  • Physiological VEGF levels are crucial for retinal homeostasis.

Purpose of the Study:

  • To investigate if inhibiting hypoxia-inducible factors (HIFs) can target pathological VEGF levels while preserving physiological amounts.
  • To explore HIF inhibition as an alternative therapeutic strategy for neovascular retinal diseases.

Main Methods:

  • Utilized the murine oxygen-induced retinopathy (OIR) model.
  • Administered topotecan and doxorubicin, known HIF inhibitors with distinct mechanisms.
  • Assessed the effects on pathological and physiological neovascularization and visual function.

Main Results:

  • Both topotecan and doxorubicin significantly inhibited pathological neovascularization in OIR.
  • Neither drug affected physiological neovascularization.
  • Topotecan administration improved visual function in OIR models.

Conclusions:

  • HIF inhibition is a promising therapeutic strategy for pathological retinal angiogenesis.
  • Targeting HIFs may offer a way to treat retinal neovascularization and neurodegeneration while maintaining essential VEGF functions.
  • This approach could lead to novel treatments for blindness-causing retinal conditions.

More Related Videos

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model10:32

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

Published on: April 2, 2021

4.2K
A Murine Model of Ischemic Retinal Injury Induced by Transient Bilateral Common Carotid Artery Occlusion05:20

A Murine Model of Ischemic Retinal Injury Induced by Transient Bilateral Common Carotid Artery Occlusion

Published on: November 12, 2020

8.1K

Related Concept Videos

Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.1K
Models of Health Promotion and Illness Prevention II01:18

Models of Health Promotion and Illness Prevention II

The person's health status fluctuates continually, varying from being in good health to becoming ill and returning to being healthy. To understand the concept of illness prevention, there are two models. First, the health-illness continuum model is a graphic representation of an individual's wellness. It states that a person is considered healthy in the absence of physical disease and the presence of good emotional health.
The agent-host-environment model states that disease results...
2.1K
Models of Health Promotion and Illness Prevention I01:25

Models of Health Promotion and Illness Prevention I

A model is a theoretical way to understand a concept or an idea. Models can overcome barriers to health regardless of diverse economic and cultural backgrounds. In addition, models make the task easier by providing different ways to approach complex issues. There are two major health promotion models: the health belief model and the health promotion model.
The health belief model (HBM) attempts to predict health-related behavior in specific belief patterns. According to the HBM, a person's...
2.8K
Induced-fit Model01:13

Induced-fit Model

Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
89.1K
Cancer Prevention02:59

Cancer Prevention

Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
8.1K
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
91.9K