Development of magnetic cross-linked peroxidase aggregates on starch as enhancement template and their application...

The cross-linked enzyme aggregates (CLEAs) technology has been considered as an efficient and carrier free immobilizing method. The importance of this method comes from its simplicity and low-cost procedure, along with the utilization probability of the crude enzyme extract. The functionalized magnetic cross linked peroxidase aggregate (MNPs-TA-CLEAs-peroxidase) was prepared successfully using different aggregation agents, followed by crosslinking with glutaraldehyde. Herein, peroxidase enzyme was extracted from cabbage leaves. The beneficial of immobilized peroxidase was confirmed by decolorization studies of different types of dyes. Even the small amount of MNPs-TA-starch-CLEAs-peroxidase was able to remove a higher content of methylene blue, Congo red, indigo carmine, and malachite green (93.18, 90.0, 68.0 and 55.0%), respectively, comparing to the free enzyme. The ability of MNPs-CLEAs-peroxidase to decolorize different types of dyes, may give a suggestion of its possible usage in environmental biotechnology, especially in wastewater treatment.