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Related Experiment Video

Updated: Jan 27, 2026

Digital Microfluidics for Automated Proteomic Processing
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Ultra-fast and automated immunohistofluorescent multistaining using a microfluidic tissue processor.

Giulia Cappi1, Diego Gabriel Dupouy2, Marta Aurelia Comino1

  • 1Lunaphore Technologies SA, EPFL Innovation Park, Building C, 1015, Lausanne, Switzerland.

Scientific Reports
|March 16, 2019
PubMed
Summary

This study introduces an automated microfluidic tissue processor for ultra-fast multiplexed immunostaining, reducing staining time per marker to just 20 minutes. This rapid, automated multistaining method integrates seamlessly into laboratory workflows.

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Area of Science:

  • Biotechnology
  • Histopathology
  • Oncology

Background:

  • Multiplexed immunostaining reveals tumor microenvironment complexity.
  • Iterative multiplexed immunostaining is time-consuming and resource-intensive, hindering routine clinical use.

Purpose of the Study:

  • To develop a fully automated and ultra-fast multistaining technique for tissue analysis.
  • To reduce the turnaround time for multiplexed immunostaining to enable routine clinical application.

Main Methods:

  • Utilized a microfluidic tissue processor (MTP) for automated immunostaining.
  • Employed tyramide signal amplification with horse-radish peroxidase (HRP) activation for staining.
  • Implemented heat-induced antibody removal for rapid sequential staining.

Main Results:

  • Achieved an ultra-fast staining time of 20 minutes per marker.
  • Demonstrated a 4-plex automated multistaining in 84 minutes with high agreement to existing methods.
  • The method is extendable to higher plex counts and chromogenic staining.

Conclusions:

  • The automated MTP offers a rapid and efficient solution for multiplexed immunostaining.
  • This technique significantly reduces turnaround time compared to conventional methods.
  • Potential to integrate -omics approaches into routine tissue diagnostics.