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Updated: Jan 27, 2026

Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs
Published on: June 14, 2012
A Practical Guide to Genome Editing Using Targeted Nuclease Technologies
Shaina N Porter1, Rachel M Levine1, Shondra M Pruett-Miller1
1Department of Cell & Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Abstract:
Genome engineering using programmable nucleases is a rapidly evolving technique that enables precise genetic manipulations within complex genomes. Although this technology first surfaced with the creation of meganucleases, zinc finger nucleases, and transcription activator-like effector nucleases, CRISPR-Cas9 has been the most widely adopted platform because of its ease of use. This comprehensive review presents a basic overview of genome engineering and discusses the major technological advances in the field. In addition to nucleases, we discuss CRISPR-derived base editors and epigenetic modifiers. We also delve into practical applications of these tools, including creating custom-edited cell and animal models as well as performing genetic screens. Finally, we discuss the potential for therapeutic applications and ethical considerations related to employing this technology in humans. © 2019 American Physiological Society. Compr Physiol 9:665-714, 2019.
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