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CRISPR/Cas9 Genome Editing

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Related Experiment Video

Updated: Jan 27, 2026

Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs

Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs

Published on: June 14, 2012

A Practical Guide to Genome Editing Using Targeted Nuclease Technologies.

Shaina N Porter¹, Rachel M Levine¹, Shondra M Pruett-Miller¹

¹Department of Cell & Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Comprehensive Physiology

|March 16, 2019

Summary

This summary is machine-generated.

Genome engineering technologies, including CRISPR-Cas9, enable precise genetic modifications. This review covers advances, applications, and ethical considerations of these powerful genome editing tools.

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Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Genome engineering allows precise genetic manipulation.
Early tools include meganucleases, zinc finger nucleases, and TALENs.
CRISPR-Cas9 is now the most adopted due to its ease of use.

Purpose of the Study:

To provide a comprehensive overview of genome engineering technologies.
To discuss major technological advances and applications.
To explore therapeutic potential and ethical implications.

Main Methods:

Review of programmable nucleases (meganucleases, ZFNs, TALENs, CRISPR-Cas9).
Discussion of CRISPR-derived base editors and epigenetic modifiers.
Exploration of applications in cell/animal models and genetic screens.

Main Results:

CRISPR-Cas9 offers significant advantages in ease of use for genome engineering.
Advances include base editors and epigenetic modifiers.
Tools facilitate custom model creation and genetic screening.

Conclusions:

Genome engineering tools have broad applications in research and biotechnology.
Therapeutic applications hold great promise but require careful ethical consideration.
The field is rapidly advancing with new tools and applications emerging.