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Apolipoprotein B is a calcium binding protein.

N Dashti, D M Lee, T Mok

Biochemical and Biophysical Research Communications

|May 29, 1986

Summary

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This study reveals that apolipoprotein B, a key component of lipoproteins, binds calcium. This finding suggests apolipoprotein B functions as a calcium-binding protein in human liver cells.

Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

Lipoproteins are crucial for lipid transport in the body.
Apolipoprotein B is a major structural protein in certain lipoproteins.
The role of calcium in lipoprotein metabolism is not fully understood.

Purpose of the Study:

To investigate the potential interaction between calcium and apolipoprotein B.
To determine if apolipoprotein B exhibits calcium-binding properties.

Main Methods:

Human hepatocarcinoma Hep G2 cells were cultured with radioactive calcium-45 ([45Ca2+]).
Secreted lipoproteins were isolated and analyzed using SDS-polyacrylamide gel electrophoresis.
Radioactivity was measured to identify calcium-binding proteins.

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Main Results:

The majority of [45Ca2+] radioactivity in secreted lipoproteins co-migrated with apolipoprotein B.
The molar ratio of incorporated [45Ca2+] to apolipoprotein B was approximately one-to-one.
Precipitation assays confirmed that only apolipoprotein B-containing lipoproteins were radioactive.

Conclusions:

Apolipoprotein B demonstrates a direct affinity for calcium ions.
These findings strongly suggest that apolipoprotein B is a calcium-binding protein.
This interaction may have implications for lipoprotein structure and function.