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Ectopic expression of miR-944 impairs colorectal cancer cell proliferation and invasion by targeting GATA binding

Jing-Tong Tang1, Jinbo Zhao1, Weiwei Sheng1

  • 1Department of Gastrointestinal Surgery & Hernia and Abdominal Wall Surgery, The First Hospital, China Medical University, Shenyang, Liaoning, China.

Journal of Cellular and Molecular Medicine
|March 16, 2019
PubMed

Insights

MicroRNA-944 acts as a tumor suppressor in colorectal cancer (CRC) by inhibiting proliferation and metastasis. Its downregulation correlates with advanced CRC stages, suggesting potential as a prognostic marker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Colorectal cancer (CRC) is a significant global health concern.
  • MicroRNA-944 (miR-944) has emerging roles in cancer progression.
  • The specific functions and mechanisms of miR-944 in CRC remain largely undefined.

Purpose of the Study:

  • To investigate the role of miR-944 in colorectal cancer.
  • To elucidate the underlying molecular mechanisms of miR-944 in CRC.
  • To assess the potential of miR-944 as a prognostic biomarker and therapeutic target for CRC.

Main Methods:

  • Real-time reverse transcription polymerase chain reaction (RT-PCR) was used to quantify miR-944 expression in 100 CRC tissues.
  • In vitro experiments assessed the effects of miR-944 overexpression and knockdown on CRC cell proliferation, migration, and invasion.
  • GATA binding protein 6 (GATA6) knockdown and Dual-Luciferase Reporter Assays were employed to explore the interaction between miR-944 and GATA6.

Main Results:

  • miR-944 expression was frequently downregulated in CRC tissues and negatively correlated with TNM stage, invasion depth, and lymph node status.
  • Overexpression of miR-944 suppressed CRC cell proliferation, migration, and invasion, while knockdown enhanced these functions.
  • miR-944 directly targets GATA6, influencing downstream proteins CRT and p-AKT, and GATA6 knockdown reversed miR-944 inhibitor-induced CRC cell changes.

Conclusions:

  • miR-944 functions as a tumor suppressor in colorectal cancer.
  • miR-944 exhibits potential as a prognostic predictor for CRC.
  • miR-944 represents a promising novel therapeutic target for colorectal cancer treatment.
Keywords:
GATA6colorectal cancerinvasionmiR-944migrationproliferation

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