Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

13.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
13.2K
Phase Diagrams02:39

Phase Diagrams

50.0K
A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
50.0K
Phase Transitions02:31

Phase Transitions

23.1K
Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to...
23.1K
Inductance: Single-Phase And Three-Phase Line01:28

Inductance: Single-Phase And Three-Phase Line

618
Understanding the inductance of transmission lines is crucial for efficient design and operation in electrical power systems. This discussion delves into the inductance characteristics of single-phase two-wire and three-phase three-wire transmission lines with equal phase spacing.
Single-Phase Two-Wire Line:
A single-phase line consists of two solid cylindrical conductors, denoted as x and y. Each conductor carries phasor currents ix and iy, respectively. Given that the sum of these currents is...
618
Capacitance: Single-Phase And Three-Phase Line01:25

Capacitance: Single-Phase And Three-Phase Line

597
In electrical power systems, understanding the capacitance of transmission lines is fundamental for efficient operation.
Single-Phase Lines
Consider a single-phase, two-wire transmission line with equal phase spacing energized by a voltage source. One conductor carries a uniform positive charge, while the other carries an equal negative charge. The capacitance C of the line can be derived from the voltage V between the conductors. For a one-meter section of the line, the capacitance is given...
597
Phase Changes01:19

Phase Changes

5.4K
Phase transitions play an important theoretical and practical role in the study of heat flow. In melting or fusion, a solid turns into a liquid; the opposite process is freezing. In evaporation, a liquid turns into a gas; the opposite process is condensation.
A substance melts or freezes at a temperature called its melting point and boils or condenses at its boiling point. These temperatures depend on pressure. High pressure favors the denser form of the substance, so typically, high pressure...
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phosphatidic Acid Reverses Obesity Induced by a High-Fat, High-Sugar Diet at the Transcriptional Level.

Genes·2025
Same author

Physiotherapy Reduces Pain and Alters Fatty Acid Biomarkers in Women with Chronic Non-Specific Low Back Pain.

International journal of women's health·2025
Same author

The Impact of Patient, Disease, and Social Determinants of Health on Receipt of Nonoperative Management for Patients With Rectal Adenocarcinoma.

American journal of clinical oncology·2025
Same author

The immediate effect of anodal tDCS over the motor cortex on postural control and cortical activation in patients with chronic low back pain: A preliminary study.

Behavioural brain research·2025
Same author

Aluminum nitride photonics for high-speed electro-optical tuning of self-injection-locked laser.

Optics letters·2025
Same author

Tumor microenvironment and Fe<sup>3+</sup>-triggered croconium nanoplatform for precise tumor location and combined type I photodynamic and photothermal amplified therapy.

Journal of colloid and interface science·2025

Related Experiment Video

Updated: Jan 27, 2026

Phase Contrast and Differential Interference Contrast DIC Microscopy
06:49

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

54.0K

Schlieren two-photon microscopy for phase-contrast imaging.

Hao Xie, Yuanlong Zhang, Linjie Kong

    Applied Optics
    |March 16, 2019
    PubMed
    Summary
    This summary is machine-generated.

    Schlieren two-photon microscopy enables simultaneous phase-contrast and fluorescence imaging on standard two-photon microscopes. This low-cost method uses modulated plates to reveal specimen phase information, enhancing live tissue imaging capabilities.

    More Related Videos

    Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery
    07:02

    Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery

    Published on: September 5, 2018

    9.9K
    In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
    10:24

    In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

    Published on: January 5, 2012

    24.8K

    Related Experiment Videos

    Last Updated: Jan 27, 2026

    Phase Contrast and Differential Interference Contrast DIC Microscopy
    06:49

    Phase Contrast and Differential Interference Contrast DIC Microscopy

    Published on: August 6, 2008

    54.0K
    Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery
    07:02

    Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery

    Published on: September 5, 2018

    9.9K
    In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
    10:24

    In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

    Published on: January 5, 2012

    24.8K

    Area of Science:

    • Biomedical Optics
    • Microscopy Techniques
    • Phase Contrast Imaging

    Background:

    • Conventional two-photon microscopes struggle to retrieve phase information, limiting comprehensive live tissue analysis.
    • Simultaneous phase-contrast and fluorescence imaging offers rich biomedical data but requires specialized equipment.

    Purpose of the Study:

    • To develop a cost-effective method for in situ phase-contrast and two-photon fluorescence imaging.
    • To integrate phase-contrast capabilities into existing two-photon microscopy platforms.

    Main Methods:

    • Proposing Schlieren two-photon microscopy, a novel technique for phase-contrast imaging on two-photon microscopes.
    • Utilizing spatially modulated fluorescence plates composed of two-photon dyes or upconversion nanoparticles.
    • Conducting theoretical analysis, simulations, and experimental validation.

    Main Results:

    • Demonstrating that fluorescence intensity fluctuations directly correlate with specimen phase gradients.
    • Confirming the feasibility and effectiveness of the Schlieren two-photon microscopy method.
    • Showing compatibility with commercial two-photon microscopes.

    Conclusions:

    • Schlieren two-photon microscopy provides a low-cost solution for simultaneous phase-contrast and fluorescence imaging.
    • This technique significantly enhances the capabilities of standard two-photon microscopes for live tissue imaging.
    • The method holds potential for widespread adoption in biomedical research.