Automating multimodal microscopy with NanoJ-Fluidics
Related Experiment Video
Updated: Jan 27, 2026

Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms
Published on: March 3, 2023
Pedro Almada1,2, Pedro M Pereira1,2,3, Siân Culley1,2,3
1MRC-Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK.
Abstract:
Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.
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