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Updated: Jan 27, 2026

Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms

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Published on: March 3, 2023

Automating multimodal microscopy with NanoJ-Fluidics.

Pedro Almada^1,2, Pedro M Pereira^1,2,3, Siân Culley^1,2,3

¹MRC-Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK.

Nature Communications

|March 16, 2019

Summary

This summary is machine-generated.

We developed NanoJ-Fluidics, an automated, open-source system using low-cost hardware and software for complex microscopy workflows. This approach simplifies multimodal imaging for reproducible, high-content cellular studies.

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Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

Area of Science:

Cellular biology
Microscopy techniques
Biotechnology

Background:

Advanced microscopy is essential for understanding cellular events.
Current workflows for combining and multiplexing microscopy are often complex and elaborate.
Need for accessible, reproducible, and automated solutions in high-content imaging.

Purpose of the Study:

To present a robust, open-source approach for automated, sequential treatment, labeling, and imaging of cells.
To simplify the implementation of high-content, multimodal imaging on various microscopes.
To demonstrate the system's capability in advanced microscopy applications.

Main Methods:

Development of NanoJ-Fluidics, an integrated system using affordable Lego hardware and ImageJ-based software.
Automation of cell treatment, labeling, and imaging sequences.
Application of the system to live-to-fixed and multiplexed super-resolution microscopy.

Main Results:

NanoJ-Fluidics enables reproducible, high-content, multimodal imaging with ease of implementation.
Demonstrated successful application in event-driven, super-resolved live-to-fixed experiments.
Validated multiplexed STORM/DNA-PAINT experiments using the automated system.

Conclusions:

NanoJ-Fluidics offers a cost-effective and reproducible solution for complex microscopy workflows.
The system enhances accessibility to advanced imaging techniques for cellular studies.
Facilitates high-content, multimodal imaging, advancing cellular event analysis.