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Updated: Jan 27, 2026

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
Published on: September 20, 2016
Oleaginous yeast for biofuel and oleochemical production
Michael Spagnuolo1, Allison Yaguchi1, Mark Blenner1
1Department of Chemical & Biomolecular Engineering, Clemson University, Clemson, SC 29634, United States.
Abstract:
Current transportation fuels derived from petroleum can also be made from microbial systems. In particular, oleaginous yeast have naturally evolved high flux pathways for fatty acids in the form of neutral lipids, which can be converted into a variety of drop-in fuels. Here, we describe the recent advances in the use of the four most popular oleaginous yeasts for making lipids and other potential fuels - Yarrowia lipolytica, Lipomyces starkeyi, Rhodosporidium toruloides, and Cutaneotrichosporon oleaginosus. The paper is divided into three major sections focusing on (1) the important natural complex phenotypes of each yeast; (2) the development of metabolic engineering tools for each yeast; and (3) demonstrations of metabolic engineering in each yeast. At the end of each section, we provide our assessment, of which yeast is most promising in the near and long term for bioenergy production.
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