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Generation and Characterization of Anti-Filovirus Nucleoprotein Monoclonal Antibodies

Md Niaz Rahim1,2, Min Wang3, Tong Wang4

  • 1Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada. mdniaz.rahim@canada.ca.

Viruses
|March 17, 2019
PubMed

Insights

Researchers developed new monoclonal antibodies (mAbs) targeting filovirus nucleoprotein (NP). These antibodies can detect multiple filovirus species, aiding in diagnostics and research for lethal hemorrhagic fevers.

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Filoviruses are a significant cause of lethal hemorrhagic fever in humans.
  • The filovirus nucleoprotein (NP) is crucial for viral replication and highly expressed during infection.

Purpose of the Study:

  • To generate and characterize a panel of monoclonal antibodies (mAbs) against filovirus nucleoprotein (NP).
  • To assess the utility of these mAbs for detecting filoviruses and understanding their replication.

Main Methods:

  • Immunization of mice with filovirus NP B-cell epitopes.
  • Screening of hybridomas using FLAG-tagged filovirus NP constructs.
  • Characterization of mAbs including isotyping, binding affinity, western blot, and immunofluorescence confocal microscopy.

Main Results:

  • Numerous anti-NP mAbs were successfully generated and characterized.
  • The mAbs exhibited varying binding affinities to different filovirus NPs.
  • Most mAbs specifically detected recombinant and wild-type NPs from multiple filovirus species (EBOV, SUDV, BDBV, MARV, TAFV, RESTV) via western blot and immunofluorescence.

Conclusions:

  • A versatile panel of monoclonal antibodies targeting filovirus NP has been developed.
  • These mAbs are effective tools for detecting various filoviruses in different formats.
  • The antibodies hold potential for diagnosing filovirus infections, studying viral replication, and investigating NP-host protein interactions.
Keywords:
Bundibugyo (BDBV)Ebola (EBOV)Marburg (MARV)Reston (RESTV) virusesSudan (SUDV)Tai Forest (TAFV)mAb (monoclonal antibody)

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