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Updated: Jan 27, 2026

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
Published on: June 5, 2020
Three-dimensional single-photon imaging through obscurants
Abstract:
We investigate the depth imaging of objects through various densities of different obscurants (water fog, glycol-based vapor, and incendiary smoke) using a time-correlated single-photon detection system which had an operating wavelength of 1550 nm and an average optical output power of approximately 1.5 mW. It consisted of a monostatic scanning transceiver unit used in conjunction with a picosecond laser source and an individual Peltier-cooled InGaAs/InP single-photon avalanche diode (SPAD) detector. We acquired depth and intensity data of targets imaged through distances of up to 24 meters for the different obscurants. We compare several statistical algorithms which reconstruct both the depth and intensity images for short data acquisition times, including very low signal returns in the photon-starved regime.
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