Related Experiment Video
Updated: Jan 27, 2026
16:03
A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms
Published on: January 11, 2015
10.0K
Method development and laboratory intercomparison of an RP-HPLC-UV method for energetic chemicals in marine tissues
Harry D Craig1, Thomas F Jenkins2, Mitch T Johnson3
1US Environmental Protection Agency, Region 10, Oregon Operations Office, 805 SW Broadway, Suite 500, Portland, OR 97205, USA.
Talanta
|March 17, 2019
Summary
A new method accurately detects 16 energetic compounds in marine tissues. This validated analytical method, using reversed-phase high-performance liquid chromatography, is suitable for environmental monitoring of explosives in seafood.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Marine Biology
Background:
- Military activities can release energetic compounds into marine environments.
- Assessing contamination in marine tissues is crucial for ecological and human health.
- Existing analytical methods require adaptation for complex biological matrices.
Purpose of the Study:
- To develop and validate a robust analytical method for determining energetic compounds in marine tissues.
- To evaluate the efficacy of sample preparation techniques, including dry-ice grinding and solid-phase extraction.
- To assess the method's performance across multiple marine species and analytes.
Main Methods:
- Reversed-phase high-performance liquid chromatography with UV detection (RP-HPLC-UV).
- Dry-ice grinding for sample homogenization.
- Solid-phase extraction (SPE) for extract cleanup.
- Method detection limit (MDL) estimation and a six-laboratory intercomparison study.
Main Results:
- Dry-ice grinding proved effective for homogenizing marine tissues.
- SPE cleanup adequately removed interfering substances.
- The modified USEPA Method 8330B demonstrated suitable detection capability, accuracy, precision, and sensitivity for 16 of 17 energetic compounds across five tissue types.
- Tetryl was found to be unstable in the tested matrices.
Conclusions:
- The developed RP-HPLC-UV method is a reliable tool for analyzing military-relevant energetic compounds in diverse marine organisms.
- The method offers a validated approach for environmental monitoring and risk assessment of explosives contamination in marine ecosystems.
- Further research may be needed to address the instability of certain compounds like tetryl.
More Related Videos
08:54Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field
Published on: August 27, 2021
1.8K
11:26Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants
Published on: May 26, 2017
11.5K
Related Concept Videos
01:30Methods of Sterilization II: Chemical Methods
9.0K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
9.0K
01:21Development of Analytical Methods
2.2K
An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
2.2K
03:50The Scientific Method
65.8K
Chemistry is an empirical science. Scientists often pose questions to understand the chemistry in everyday life and seek answers to these questions. To achieve this, scientists follow a definitive series of steps that together make up the Scientific Method. This approach involves making observations, asking questions, building a hypothesis, conducting experiments, analyzing results, and forming a conclusion.
65.8K
01:32The Scientific Method
263.2K
The scientific method is a detailed, empirical problem-solving process used by biologists and other scientists. This iterative approach involves formulating a question based on observation, developing a testable potential explanation for the observation (called a hypothesis), making and testing predictions based on the hypothesis, and using the findings to create new hypotheses and predictions.
Generally, predictions are tested using carefully-designed experiments. Based on the outcome of these...
Generally, predictions are tested using carefully-designed experiments. Based on the outcome of these...
263.2K
02:35Energetics of Solution Formation
7.4K
The formation of a solution is an example of a spontaneous process, which is a process that occurs under specified conditions without energy from some external source.
When the strengths of the intermolecular forces of attraction between solute and solvent species in a solution are no different than those present in the separated components, the solution is formed with no accompanying energy change. Formation of the solution requires the solute–solute and solvent–solvent...
When the strengths of the intermolecular forces of attraction between solute and solvent species in a solution are no different than those present in the separated components, the solution is formed with no accompanying energy change. Formation of the solution requires the solute–solute and solvent–solvent...
7.4K
01:29Methods of Sterilization I: Physical Methods
23.5K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
23.5K