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Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array

Luis A Tortajada-Genaro1, Eric Seiti Yamanaka2, Ángel Maquieira1

  • 1Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Valencia, Spain; Unidad Mixta UPV-La Fe, Nanomedicine and Sensors, IIS La Fe, Valencia, Spain.

Talanta
|March 17, 2019
PubMed
Summary
This summary is machine-generated.

Consumer electronics enable compact DNA biomarker testing for point-of-care diagnostics. This method uses loop-mediated isothermal amplification and on-chip hybridization with everyday devices for accurate genetic analysis.

Keywords:
Compact discIsothermal DNA amplificationPoint-of-care testingScannerSingle-nucleotide polymorphismSmartphone

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Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Consumer Electronics in Healthcare

Background:

  • Consumer electronic devices offer potential for developing portable and cost-effective biosensing systems.
  • Point-of-care testing (POCT) for DNA biomarkers requires sensitive and accessible detection methods.
  • Existing POCT methods often rely on expensive or specialized equipment, limiting widespread adoption.

Purpose of the Study:

  • To develop and integrate a DNA biomarker detection method using consumer electronics for point-of-care applications.
  • To evaluate the performance of common consumer devices as readers for genetic analysis.
  • To demonstrate the feasibility of using low-cost, user-friendly technology for genotyping single nucleotide polymorphisms.

Main Methods:

  • Developed a method for single nucleotide polymorphism discrimination based on loop-mediated isothermal amplification (LAMP) and on-chip hybridization.
  • Integrated the assay with consumer optical devices: USB digital microscope, flatbed scanner, smartphone, and DVD drive.
  • Utilized colorimetric staining for signal detection and compared the performance of the four consumer electronic readers.

Main Results:

  • Achieved high amplification yields (8 orders) within 45 minutes using LAMP.
  • Demonstrated sensitive and precise measurements with high signal-to-noise ratios and 10-μm resolution.
  • Confirmed excellent analytical performance in human samples with a low detection limit (100 genomic DNA copies) and high reproducibility (<15%).
  • Successfully genotyped a specific single nucleotide polymorphism (in the GRIK4 gene) with results agreeing with sequencing data.
  • Assays were cost-effective (< €10/test) and reproducible.

Conclusions:

  • Consumer electronic devices can serve as effective, low-cost readers for DNA biomarker detection at the point-of-care.
  • The integrated platform offers a user-friendly, portable, and competitive alternative to traditional fluorescence scanners.
  • This technology facilitates the implementation of genetic testing in minimally specialized clinical laboratories, advancing telemedicine capabilities.