Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

80.9K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
80.9K
Membrane Lipids01:32

Membrane Lipids

34.1K
Lipids are an essential component of all biological membranes. The average lipid content in mammalian membranes is 50%, though it can be as low as 20% in the inner mitochondrial membrane or as high as 80% in the myelin sheath present around the nerve cells.
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
34.1K
Membrane Proteins01:30

Membrane Proteins

30.4K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
30.4K
Quantitative Analysis01:12

Quantitative Analysis

1.4K
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
1.4K
What are Lipids?01:38

What are Lipids?

219.9K
Overview
219.9K
Structure of Lipids03:38

Structure of Lipids

98.6K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
98.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Raft Affinity and Membrane Modulation of Docosahexaenoic Acid-Containing Phospholipids Revealed by Comparative Analysis Using Fluorescently Labeled Lipids.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Structural Analysis of Gel-Phase-Like Subdomains Formed in Lipid-Raft-Mimetic Bilayers.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Corrigendum to "Intermolecular interactions of perfluoroalkyl acids with human heart-type fatty acid-binding protein" [Int. J. Biol. Macromol. 369 (2026) 152710].

International journal of biological macromolecules·2026
Same author

Intermolecular interactions of perfluoroalkyl acids with human heart-type fatty acid-binding protein.

International journal of biological macromolecules·2026
Same author

The dynamic basis of G-protein recognition and activation by a GPCR.

Nature·2026
Same author

Quantitative Mapping of the Lipid Nanoenvironment around Transmembrane Proteins in Living Cells.

ACS nano·2026

Related Experiment Video

Updated: Jan 27, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

9.4K

A concise method for quantitative analysis of interactions between lipids and membrane proteins.

Masataka Inada1, Masanao Kinoshita1, Ayumi Sumino2

  • 1Department of Chemistry, Faculty of Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Analytica Chimica Acta
|March 17, 2019
PubMed
Summary

Researchers developed a new surface plasmon resonance (SPR) method to quantitatively analyze lipid-membrane protein (MP) interactions. This SPR method successfully identified specific lipid-MP binding, enabling a better understanding of membrane protein functions.

Keywords:
BacteriorhodopsinLipidMembrane proteinQuantitative interaction analysisSelf-assembled monolayerSurface plasmon resonance

More Related Videos

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
07:26

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

Published on: January 9, 2012

24.1K
Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

4.5K

Related Experiment Videos

Last Updated: Jan 27, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

9.4K
High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
07:26

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

Published on: January 9, 2012

24.1K
Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

4.5K

Area of Science:

  • Biochemistry and Biophysics
  • Membrane Protein Research
  • Lipid-Protein Interactions

Background:

  • Understanding lipid-membrane protein (MP) interactions is crucial for elucidating MP functions.
  • Existing experimental methods for analyzing these interactions are limited and lack convincing results.
  • A need exists for a precise and efficient method to quantitatively study lipid-MP interactions.

Purpose of the Study:

  • To develop a concise surface plasmon resonance (SPR)-based method for quantitative analysis of lipid-MP interactions.
  • To utilize a self-assembled monolayer (SAM) with a C6-chain on a sensor chip for MP immobilization.
  • To examine the interaction between bacteriorhodopsin (bR) as an MP and various lipids.

Main Methods:

  • Developed an SPR-based assay using a C6-chain self-assembled monolayer (SAM) coated sensor chip.
  • Immobilized bacteriorhodopsin (bR), a membrane protein, onto the SAM-modified sensor chip.
  • Quantitatively analyzed the binding of various lipids to the immobilized bR using SPR.

Main Results:

  • The C6-SAM surface enhanced MP immobilization by 10-fold compared to a hydrophilic CM5 chip, providing a partial membrane environment.
  • Atomic Force Microscopy (AFM) confirmed bR molecules were exposed on the SAM surface, accessible to lipids.
  • Identified S-TGA-1, a halobacterium-derived glycolipid, as having high specificity for bR, with a nanomolar dissociation constant.

Conclusions:

  • The developed SPR method with C6-SAM is effective for quantitative analysis of lipid-MP interactions.
  • The method successfully reproduced and quantitatively evaluated specific lipid-bR recognition, validating its utility.
  • This technique provides a valuable tool for studying lipid-protein interactions in membrane protein research.