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Updated: Jan 27, 2026
Peptide-based Identification of Functional Motifs and their Binding Partners
Published on: June 30, 2013
Identification of FAM96B as a novel selenoprotein W binding partner in the brain
Ping Chen1, Chao Wang2, Qing Liu3
1Department of Biochemical Engineering, Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, Nanyang Institute of Technology, 473004, Nanyang, China.
Abstract:
Selenoprotien W (SelW) plays a key role in brain development, although the exact biological function and mechanisms remain unclear. We performed a yeast two-hybrid screen on a human fetal brain cDNA library and identified FAM96B as a novel binding partner of SelW. FRET analyses confirmed the interaction between SelW' and FAM96B. The mutated SelW' construct was cloned and overexpressed in E. coli, and a pull-down assay verified a direct interaction between SelW' and FAM96B. Finally, Co-Immunoprecipitation on murine brain tissue proteins demonstrated an endogenous interaction between the two proteins in the brain. Taken together, our findings prove a direct interaction between SelW and FAM96B, which may provide new insights into the role of SelW in brain development and neurodegenerative diseases.
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