DamID as a versatile tool for understanding gene regulation
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Updated: Jan 27, 2026

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
Published on: October 25, 2017
Gabriel N Aughey1, Seth W Cheetham2, Tony D Southall3
1Department of Life Sciences, Imperial College London, Sir Ernst Chain Building, London, SW7 2AZ, UK.
Abstract:
The interaction of proteins and RNA with chromatin underlies the regulation of gene expression. The ability to profile easily these interactions is fundamental for understanding chromatin biology in vivo DNA adenine methyltransferase identification (DamID) profiles genome-wide protein-DNA interactions without antibodies, fixation or protein pull-downs. Recently, DamID has been adapted for applications beyond simple assaying of protein-DNA interactions, such as for studying RNA-chromatin interactions, chromatin accessibility and long-range chromosome interactions. Here, we provide an overview of DamID and introduce improvements to the technology, discuss their applications and compare alternative methodologies.
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