Related Experiment Video
Updated: Jan 27, 2026

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique
Published on: January 10, 2013
Fluorescent Polymer Dots for Tracking SKOV3 Cells in Living Mice with Probe-Based Confocal Laser Endomicroscopy
Yufan Zhang1, Yao Li1, Yixiao Guo1
1Shanghai Med-X Engineering Center for Medical Equipment and Technology, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
Purpose:
Probe-based confocal laser endomicroscopy (pCLE) is a novel technique allowing real-time and high-resolution imaging in vivo. It provides microscopic images and increases the penetration depth of tissues compared with conventional white light endoscopy. The aim of the present study was to track ovarian cancer cells in organs by fluorescent polymer dots based on pCLE.
Procedures:
SKOV3-mCherry cells were incubated with polymer dots for 24 h in a serum-free culture medium. Labeled cells were administrated to nude mice via intravenous, intraperitoneal, and lymph node injection. The fluorescent signals of labeled cells in organs were observed by pCLE. Furthermore, the results were confirmed by frozen section analysis.
Results:
pCLE displayed fluorescence signals of labeled cells in the vessels of organs. Besides, the accumulations of labeled cells visualized in detoxification organs like the spleen and kidney were increased with time.
Conclusions:
In this article, we present a real-time and convenient method for tracking SKOV3-mCherry in living mice by combined fluorescent polymer dots with pCLE.
More Related Videos
Related Concept Videos
Polymers
Polymers
Confocal Fluorescence Microscopy
Dot Product
In engineering, the dot product of any two vectors is the product of the magnitudes of the vectors and the cosine of the angle between them. It is denoted by a dot symbol between the two vectors.
Consider a vehicle pulling an object along the ground using a rope. If the rope makes an angle with the horizontal axis, the work done can be calculated using the dot product of the force applied and the object's displacement.
The dot...
Dot Product: Problem Solving
Identify the problem: Start by reading the problem and...
Scalar Product (Dot Product)
The scalar product of two vectors is obtained by multiplying...

