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Generation of two transgene-free human iPSC lines from CD133+ cord blood cells

Estibaliz Arellano-Viera1, Lorea Zabaleta1, Julio Castaño1

  • 1Inbiomed Foundation, San Sebastian, Spain.

Stem Cell Research
|March 17, 2019
PubMed

Insights

Two human induced pluripotent stem cell (iPSC) lines were generated from umbilical cord blood. These cell lines, PRYDi-CB5 and PRYDi-CB40, have been fully characterized for research applications.

Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Genetics

Background:

  • Human induced pluripotent stem cells (iPSCs) are crucial for disease modeling and regenerative therapies.
  • Umbilical cord blood (CB) is a valuable source of hematopoietic stem cells.
  • Generating and characterizing novel iPSC lines is essential for expanding research capabilities.

Purpose of the Study:

  • To generate and fully characterize two new human induced pluripotent stem cell (iPSC) lines.
  • To utilize CD133+ cells from umbilical cord blood for iPSC generation.
  • To establish well-defined iPSC lines for future research.

Main Methods:

  • Isolation of CD133+ cells from human umbilical cord blood.
  • Reprogramming of CD133+ cells into iPSCs using non-integrative Sendai virus.
  • Comprehensive characterization of the generated iPSC lines (e.g., pluripotency, differentiation, genetic stability).

Main Results:

  • Successfully generated two distinct human iPSC lines, designated PRYDi-CB5 and PRYDi-CB40.
  • Confirmed the pluripotency of both iPSC lines.
  • Demonstrated the successful reprogramming of CD133+ cells from umbilical cord blood.

Conclusions:

  • The generated iPSC lines (PRYDi-CB5 and PRYDi-CB40) are fully characterized and suitable for further research.
  • This study provides valuable new human iPSC resources derived from umbilical cord blood.
  • Non-integrative Sendai virus is an effective method for generating clinical-grade iPSCs.

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