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Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
Published on: July 1, 2018
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Non-structural Functions of Hordeivirus Capsid Protein Identified in Plants Infected by a Chimeric Tobamovirus
S S Makarova1, A V Makhotenko1, A V Khromov1
1Lomonosov Moscow State University, Biological Faculty, Moscow, 119991, Russia.
Biochemistry. Biokhimiia
|March 18, 2019
Summary
Barley stripe mosaic virus capsid protein (CP) performs non-structural roles in tobamovirus infection, including systemic transport and pathogenicity. This suggests conserved functions across related plant virus families.
Area of Science:
- Plant virology
- Molecular biology
- Protein function
Background:
- Capsid proteins (CPs) of (+)RNA plant viruses are crucial for virion formation and viral infection.
- Tobamoviral CPs mediate systemic transport, host interactions, and pathogenicity.
- Hordeiviral CPs share structural similarities with tobamoviral CPs, but their non-structural functions remain uncharacterized.
Purpose of the Study:
- To investigate the non-structural functions of the barley stripe mosaic virus (BSMV) CP.
- To determine if BSMV CP can substitute for tobamoviral CP functions in a heterologous viral system.
Main Methods:
- Genetic modification of turnip vein clearing virus (TVCV) genome by replacing its CP gene with BSMV CP or its mutants.
- Assaying the function of BSMV CP in systemic transport, hypersensitive response elicitation, and pathogenicity in plants.
Main Results:
- BSMV CP effectively replaced TVCV CP in all stages of viral infection.
- BSMV CP mediated long-distance transport of the chimeric tobamovirus.
- BSMV CP acted as a hypersensitive response elicitor and influenced pathogenicity.
- A C-terminally truncated BSMV CP in the chimeric tobamovirus enhanced infectivity and resulted in atypical virion formation.
Conclusions:
- Hordeiviral CPs possess non-structural functions analogous to those of tobamoviral CPs.
- The BSMV CP can functionally substitute for the TVCV CP in a heterologous tobamovirus system.
- Conserved non-structural roles of CPs may exist within the Virgaviridae family.
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