Electrochemical sensing of nicotine using CuWO₄ decorated reduced graphene oxide immobilized glassy carbon electrode

A novel and selective electrochemical sensing of nicotine is studied using copper tungstate decorated reduced graphene oxide nanocomposite (CuWO₄/rGO) nafion (Nf) immobilized GC electrode (GCE). The CuWO₄/rGO nanocomposite is synthesized using sonication method and characterized by HR-TEM (High resolution transmission electron microscopy), SEM (Scanning electron microscopy), FT-IR (Fourier transform infrared spectroscopy), SAED (Selected area of electron diffraction pattern), XRD (X-ray diffraction), Raman spectroscopy, Thermo gravimetric analysis (TGA) and EDX (Energy dispersive X-ray diffraction) techniques. The CuWO₄/rGO/Nf immobilized GCE shows better electrocatalytic response for the detection of nicotine as compared to bare GCE. A better selectivity and sensitivity is achieved using CuWO₄/rGO/Nf immobilized GCE to detect 0.1 µM nicotine in the presence of 100-fold excess concentrations of different interferents. The present CuWO₄/rGO/Nf immobilized GCE electrochemical sensor exhibits an ample range of sensing from 0.1 µM to 0.9 µM and the low detection limit is found to be 0.035 µM (S/N = 3). Comparable results are achieved for the determination of nicotine in various real samples such as cigarettes (Gold flake and Wills) and urine samples with improved recoveries.