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Optimized Management of Endovascular Treatment for Acute Ischemic Stroke
Published on: January 18, 2018
Plasma Levels of miR-125b-5p and miR-206 in Acute Ischemic Stroke Patients After Recanalization Treatment: A
Xin-Wei He1, Yan-Hui Shi1, Rong Zhao1
1Department of Neurology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Clinical Research Centre, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Introduction:
Multiple microRNAs (miRNAs) participate in the response to hypoxic/ischemic and ischemia-reperfusion events. However, the expression of these miRNAs in circulation from patients with acute ischemic stroke (AIS) receiving recanalization treatment has not been examined, and whether they are associated with the severity and outcome of stroke is still unknown.
Materials And Methods:
In this prospective cohort study, plasma levels of miR-125b-5p, miR-15a-3p, miR-15a-5p, and miR-206 were measured at 24 hours after thrombolysis with or without endovascular treatment in 94 patients with AIS, as determined by qRT-PCR. Stroke severity was assessed based on National Institutes of Health Stroke Scale (NIHSS) score and infarct lesion. Intracranial haemorrhage (ICH) was recorded. An unfavorable outcome was defined as a modified Rankin Scale score greater than 2 at day 90 after stroke.
Results:
miR-125b-5p and miR-206 levels were correlated with NIHSS scores (P = .014 and P = .002) and cerebral infarction volumes (P = .025 and P = .030). miR-125b-5p levels were significantly higher in patients with an unfavorable outcome than in patients with a favorable outcome (P = .002) and showed good diagnostic accuracy in discriminating the presence of an unfavorable outcome (area under the curve .735, 95% confidence interval .623-.829, P < .001). No association was found between different miRNAs and ICH.
Conclusions:
In AIS patients after thrombolysis with or without endovascular treatment, miR-125b-5p is a novel prognostic biomarker highly associated with an unfavorable outcome. miR-125b-5p and miR-206 levels are associated with stroke severity.
Insights
MicroRNA (miRNA) levels in acute ischemic stroke (AIS) patients after treatment were analyzed. Higher miR-125b-5p levels indicate a poor outcome, while miR-125b-5p and miR-206 correlate with stroke severity.
Area of Science:
- Biochemistry
- Molecular Biology
- Neurology
Background:
- MicroRNAs (miRNAs) are implicated in cellular responses to hypoxia and ischemia.
- Circulating miRNA expression in acute ischemic stroke (AIS) patients undergoing recanalization therapy remains understudied.
- The association of these miRNAs with stroke severity and patient outcomes is largely unknown.
Purpose of the Study:
- To investigate the expression of specific circulating miRNAs in AIS patients post-recanalization treatment.
- To determine the relationship between these miRNA levels and stroke severity.
- To evaluate the prognostic value of these miRNAs for patient outcomes.
Main Methods:
- A prospective cohort study involving 94 AIS patients treated with thrombolysis and/or endovascular therapy.
- Plasma levels of miR-125b-5p, miR-15a-3p, miR-15a-5p, and miR-206 were quantified using qRT-PCR 24 hours post-treatment.
- Stroke severity was assessed using the National Institutes of Health Stroke Scale (NIHSS) and infarct volume; outcomes were evaluated using the modified Rankin Scale (mRS) at 90 days.
Main Results:
- miR-125b-5p and miR-206 levels positively correlated with NIHSS scores and cerebral infarction volumes.
- Significantly elevated miR-125b-5p levels were observed in patients with unfavorable outcomes (mRS > 2) compared to those with favorable outcomes.
- miR-125b-5p demonstrated good diagnostic accuracy for predicting unfavorable outcomes (AUC = 0.735).
Conclusions:
- Circulating miR-125b-5p serves as a novel prognostic biomarker for unfavorable outcomes in AIS patients following recanalization therapy.
- Plasma levels of miR-125b-5p and miR-206 are associated with the severity of acute ischemic stroke.
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