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Assembly and Characterization of Polyelectrolyte Complex Micelles
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Early hMSC morphology and proliferation on model polyelectrolyte multilayers.

Ivan Ding1, Jenna A Walz2, Charles R Mace2

  • 1Department of Chemical Engineering, University of Massachusetts Lowell, One University Ave, Lowell, MA, 01854, United States.

Colloids and Surfaces. B, Biointerfaces
|March 18, 2019
PubMed
Summary
This summary is machine-generated.

Polyelectrolyte multilayers (PEMs) coatings influence human mesenchymal stem cell (hMSC) attachment and proliferation. Initial layer choice impacts surface properties, but complex interactions, not just roughness or energy, dictate cell behavior.

Keywords:
Cell culture substratePolyelectrolyte multilayerSurface energySurface topographyhMSC

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Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Polyelectrolyte multilayers (PEMs) are versatile coatings for biomedical applications.
  • PEM surface properties, including roughness and energy, are tunable via layer selection.
  • The initial layer significantly influences PEM characteristics and subsequent cell interactions.

Purpose of the Study:

  • To investigate the effect of the initial layer material on PEM properties.
  • To evaluate the impact of different PEM coatings on human mesenchymal stem cell (hMSC) attachment and proliferation.
  • To correlate surface properties with cellular responses to PEM coatings.

Main Methods:

  • Fabrication of 5-bilayer PEMs using poly(diallyldimethylammonium chloride) (PDADMAC) and poly(sodium 4-styrenesulfonate) (PSS) on gold-sputtered quartz.
  • Varied the first deposited layer and added a final poly-l-lysine (PLL) layer for all samples.
  • Characterized surface roughness and surface energy; cultured hMSCs for short-term (90 min) and long-term (4 days) analysis.

Main Results:

  • Initial layer selection altered PEM roughness.
  • All PEMs exhibited increased dispersive and decreased polar surface energy compared to controls.
  • Enhanced hMSC attachment was observed on PDADMAC-initiated PEMs initially, but proliferation was reduced after 4 days compared to controls.

Conclusions:

  • PEM surface properties (roughness, energy) do not solely predict hMSC behavior.
  • Complex surface interactions, potentially involving charge distribution and functional groups, govern cell attachment and proliferation.
  • PEMs may negatively impact long-term hMSC proliferation despite favorable initial attachment.