Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:46

Epigenetic Regulation

33.7K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.7K
Epigenetic Regulation01:37

Epigenetic Regulation

3.9K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.9K
Aging01:26

Aging

667
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
667
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

3.5K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
3.5K
Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

714
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
714
Changes in the Appendicular Skeleton with Age01:09

Changes in the Appendicular Skeleton with Age

3.5K
The upper and lower limb initially develops as a small bulge called a limb bud, which appears on the lateral side of the early embryo. The upper limb bud appears near the end of the fourth week of development, with the lower limb bud appearing shortly after.
Initially, the limb buds consist of a core of mesenchyme covered by a layer of ectoderm. The ectoderm at the end of the limb bud thickens to form a narrow crest called the apical ectodermal ridge. This ridge stimulates the underlying...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term outdoor air pollution and risk of ovarian and endometrial cancers in a large prospective cohort.

Journal of exposure science & environmental epidemiology·2026
Same author

Residential proximity to Superfund sites and breast cancer incidence in a nationwide cohort.

Environmental epidemiology (Philadelphia, Pa.)·2026
Same author

History of Cannabis Smoking and Subjective Cognitive Complaints in Older Women.

Neuroepidemiology·2026
Same author

Association between noise pollution exposure and incident breast cancer in the Sister Study.

Environmental research·2026
Same author

Air pollution and breast cancer incidence in a United States-wide prospective cohort study: Examining sensitive periods of exposure.

Environment international·2025
Same author

Environmental factors influencing hormone receptor positive breast cancer incidence: integrating chemical signatures from dust wipes with self-reported sources of exposure.

Journal of exposure science & environmental epidemiology·2025

Related Experiment Video

Updated: Jan 27, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.4K

Shift work, DNA methylation and epigenetic age.

Alexandra J White1, Jacob K Kresovich1, Zongli Xu1

  • 1Epidemiology Branch, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA.

International Journal of Epidemiology
|March 18, 2019
PubMed
Summary
This summary is machine-generated.

Shift work accelerates biological aging, as measured by DNA methylation (DNAm) age. This epigenetic age acceleration is linked to long-term negative health effects and specific gene methylation changes.

Keywords:
DNA methylationShift-work scheduleageingcircadian rhythmepigenomics

More Related Videos

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

24.2K
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
09:06

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Published on: October 24, 2018

11.2K

Related Experiment Videos

Last Updated: Jan 27, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.4K
Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

24.2K
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
09:06

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Published on: October 24, 2018

11.2K

Area of Science:

  • Epigenetics
  • Occupational Health
  • Chronobiology

Background:

  • Shift work is linked to increased morbidity and mortality.
  • Biological age, assessed via DNA methylation (DNAm), can quantify health risks.
  • Epigenetic age acceleration may reflect shift work's impact on aging.

Purpose of the Study:

  • To investigate the association between shift work history and epigenetic age acceleration.
  • To identify specific DNA methylation changes related to shift work.

Main Methods:

  • DNA methylation (DNAm) was measured in 2574 non-Hispanic White women (aged 35-74).
  • Three epigenetic clocks calculated DNAm age; age acceleration was derived from residuals.
  • Linear regression and epigenome-wide association studies (EWAS) analyzed shift work's impact.

Main Results:

  • Shift work was associated with higher epigenetic age acceleration.
  • Longer duration of night shift work showed the strongest association (β=3.16).
  • EWAS identified differential DNAm at 66 (overall) and 85 (night) CpG sites, including ZFHX3.

Conclusions:

  • Shift work is associated with epigenetic age acceleration and differential DNA methylation.
  • These findings suggest long-term negative health consequences of shift work.