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Related Experiment Videos

Decrease in adherence of bacteria and yeasts to human mucosal epithelial cells by noxythiolin in vitro.

S P Gorman, D F McCafferty, A D Woolfson

    The Journal of Applied Bacteriology
    |April 1, 1986
    PubMed
    Summary
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    The antimicrobial noxythiolin effectively reduced the adherence of Candida albicans, Escherichia coli, and Staphylococcus saprophyticus to epithelial cells. This anti-adherence effect was observed in both microbial growth phases and forms, unlike its degradation products.

    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Pharmacology

    Background:

    • Microbial adherence to host cells is a crucial step in many infections.
    • Candida albicans, Escherichia coli, and Staphylococcus saprophyticus are common causes of buccal, vaginal, and urinary tract infections, respectively.
    • Understanding and inhibiting microbial adherence can lead to novel therapeutic strategies.

    Purpose of the Study:

    • To quantify the adherence of specific microbial isolates to relevant epithelial cells.
    • To investigate the effect of the antimicrobial agent noxythiolin on microbial adherence.
    • To compare the efficacy of noxythiolin with its degradation products in preventing adherence.

    Main Methods:

    • Quantification of microbial adherence using light microscopy.

    Related Experiment Videos

  • Testing adherence of Candida albicans (buccal/vaginal isolates) to buccal epithelial cells.
  • Testing adherence of Escherichia coli and Staphylococcus saprophyticus (urine isolates) to uroepithelial cells.
  • Evaluating the impact of noxythiolin on microbes in exponential and stationary growth phases.
  • Assessing the effect of treating either epithelial cells or microorganisms with noxythiolin.

    • Main Results:

    • Noxythiolin significantly reduced the adherence of Candida albicans, Escherichia coli, and Staphylococcus saprophyticus to their respective host cells.
    • The anti-adherence effect of noxythiolin was observed regardless of the microbial growth phase (exponential or stationary).
    • Both blastospore and pseudohyphal forms of Candida albicans showed reduced adherence after noxythiolin treatment.
    • Treatment of either the epithelial cells or the microorganisms with noxythiolin resulted in decreased adherence.
    • Formaldehyde and N-methylthiourea, degradation products of noxythiolin, did not exhibit any anti-adherence effect.

    Conclusions:

    • Noxythiolin is an effective agent for reducing the adherence of key pathogenic microorganisms to host epithelial cells.
    • The anti-adherence mechanism of noxythiolin is distinct from its degradation products, suggesting a specific mode of action.
    • These findings highlight the potential of noxythiolin as a therapeutic agent to prevent or treat infections by inhibiting microbial colonization.