Physicochemical and antioxidant potential of polysaccharides sequentially extracted from Amana edulis

Amana edulis polysaccharides (AEPs) specifically HBSS, CHSS, DASS, and CASS were sequentially extracted with four different solvents. The present study characterized the AEPs with particular focus on their physicochemical and anti-oxidant based functional properties. Initially, monosaccharide analysis revealed arabinose (31.7%, 32.5%, 36.5%) as the main sugar in HBSS, CHSS, and DASS whereas, galactose (31.4%) in CASS besides their respective molecular weights of 6.29 × 10², 1.5 × 10², 8.1 × 10², and 2.6 × 10³ kD. HBSS showed the maximum solubility, while, CASS was observed for higher foam capacity and foam stability. Among all the fractions, DASS was observed with higher thermal stability. HBSS showed the highest ABTS⁺ scavenging activity. HBSS and CASS had higher DPPH and OH^- scavenging activities. DASS depicted the highest chelation and reducing ability. To summarize, these polysaccharides fractions may be further utilized for their enormous prospective in functional foods preparation.