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Related Experiment Video

Updated: Jan 27, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

Tracing MYC Expression for Small Molecule Discovery.

Jutta Steinberger¹, Francis Robert¹, Maxime Hallé¹

¹Department of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada.

Cell Chemical Biology

|March 19, 2019

Summary

This summary is machine-generated.

Developing new therapeutic strategies is crucial for targeting MYC (pronounced “my-seek”). Researchers created a novel assay using CRISPR/Cas9 to monitor MYC expression, identifying potent inhibitors like cardiac glycosides.

Keywords:

CRISPR/Cas9 MYC expression chemical biology drug discovery drug repurposing genome engineering multiple myelomas phenotype-based assay target identification

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Area of Science:

Oncology
Molecular Biology
Drug Discovery

Background:

Targeting MYC, a key oncogene, remains challenging for cancer therapeutics.
Existing methods for monitoring MYC expression are insufficient for drug screening.

Purpose of the Study:

To develop a novel phenotype-based assay for monitoring MYC expression in multiple myeloma cells.
To identify novel MYC expression modulators using a high-throughput screening approach.

Main Methods:

Engineered co-expression of MYC and an unstable variant of GFP using CRISPR/Cas9 at the endogenous MYC locus.
Implemented a fluorescence readout as a surrogate for MYC expression.
Conducted a pilot screen of approximately 10,000 compounds.

Main Results:

Successfully established a CRISPR/Cas9-engineered MYC expression assay.
Identified cardiac glycosides and cytoskeletal disruptors as potent inhibitors of MYC expression.
Demonstrated the utility of this assay for identifying gene expression modulators.

Conclusions:

CRISPR/Cas9 engineering provides a powerful platform for developing phenotype-based drug discovery assays.
The developed assay enables efficient screening for MYC expression modulators.
Novel classes of compounds, including cardiac glycosides, show potential for targeting MYC-driven cancers.