An anti-inflammatory C-stiryl iridoid from Camptosorus sibiricus Rupr

Area of Science:

• Phytochemistry
• Pharmacology
• Natural Products Chemistry

Background:

• Camptosorus sibiricus (Aspleniaceae) is a fern species with potential medicinal properties.
• Natural products are a rich source of bioactive compounds for drug discovery.

Purpose of the Study:

• To isolate and characterize chemical constituents from Camptosorus sibiricus.
• To evaluate the anti-inflammatory potential of isolated compounds.

Main Methods:

• Phytochemical investigation involving isolation and purification techniques.
• Structural elucidation using spectroscopic methods (1D and 2D NMR).
• In vitro anti-inflammatory assay using lipopolysaccharide-induced RAW 264.7 macrophages.

Main Results:

• A new iridoid glycoside, camptoside (1), was identified.
• Three known compounds: dehydrodiconiferyl alcohol-9'-O-β-d-glucopyranoside (2), aesculetin (3), and vajicoside (4) were isolated.
• Compounds 1, 2, and 3 showed inhibitory activity against nitric oxide production with IC50 values of 11.2 μM, 8.3 μM, and 9.4 μM, respectively.

Conclusions:

• Camptosorus sibiricus is a source of bioactive iridoid glycosides.
• Camptoside and related compounds possess anti-inflammatory properties.
• The isolated compounds may serve as leads for developing novel anti-inflammatory agents.