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Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
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Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges
Guo-Qing Song1, Humberto Prieto2, Vladimir Orbovic3
1Department of Horticulture, Plant Biotechnology Resource and Outreach Center, Michigan State University, East Lansing, MI, United States.
Frontiers in Plant Science
|March 19, 2019
Summary
Genetic engineering advances fruit crop improvement by overcoming breeding challenges. Recent progress in Agrobacterium-mediated transformation and genome editing offers new strategies for crop enhancement.
Area of Science:
- Agricultural Science
- Plant Biotechnology
- Genetics
Background:
- Conventional breeding of woody fruit crops is hindered by long generation times, heterozygosity, and sexual incompatibility.
- Genetic engineering offers a powerful alternative for precise gene manipulation in fruit crops.
- Significant progress has been made in Agrobacterium-mediated transformation for various fruit crops.
Purpose of the Study:
- To review recent advancements (past 5 years) in genetic transformation of woody fruit crops.
- To highlight reproducible transformation protocols and strategies.
- To discuss the potential and challenges of genome editing in fruit crop improvement.
Main Methods:
- Review of published literature on genetic transformation and genome editing in fruit crops.
- Analysis of transformation strategies including direct scion transformation and GM rootstocks.
- Evaluation of genome editing technologies and their application in fruit crops.
Main Results:
- Agrobacterium-mediated transformation has enabled improvements in fruit quality, biotic/abiotic tolerance, and gene function analysis.
- Transgrafting using genetically modified (GM) rootstocks presents a method for producing non-GM fruit products.
- Genome editing shows promise but faces challenges in regeneration and efficiency, particularly in polyploid fruit crops.
Conclusions:
- Effective transient transformation and efficient regeneration are crucial for advancing genome editing applications in fruit crops.
- Continued research is needed to overcome regeneration barriers and enhance gene-editing efficiency in polyploid fruit species.
- Genetic engineering and genome editing hold significant potential for accelerating the improvement of woody fruit crops.
Keywords:
Agrobacteriumgenetic engineeringgenetic transformationgenome editingwoody fruit and nut cropsMore Related Videos
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