Related Experiment Video

Updated: Jan 27, 2026

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
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A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

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Investigation of Key Genes and Pathways in Inhibition of Oxycodone on Vincristine-Induced Microglia Activation by

Wei Liu1, Jishi Ye2, Hong Yan1

  • 1Department of Anesthesiology, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.

Disease Markers
|March 19, 2019
PubMed
Abstract

Insights

This study identifies genes and pathways involved in chemotherapy-induced neuropathic pain. Oxycodone was found to reduce inflammation in microglia, suggesting a potential therapeutic role in managing chemotherapy side effects.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Genomics

Background:

  • Chemotherapy-induced neuropathic pain (CINP) mechanisms are not fully understood.
  • Microglia activation is a key factor in neuropathic pain development.
  • Vincristine is a common chemotherapeutic agent known to cause neuropathic pain.

Purpose of the Study:

  • To identify differentially expressed genes (DEGs) and pathways in vincristine-induced neuropathic pain using bioinformatics.
  • To investigate the effects of oxycodone on these DEGs in a vincristine-induced microglia activation model.

Main Methods:

  • Microarray analysis (GSE53897) to identify DEGs in vincristine-treated rats.
  • Bioinformatic tools (ToppGene, GO, pathway enrichment, PPI network) for DEG prioritization and analysis.
  • In vitro modeling of vincristine-induced microglia activation, followed by real-time PCR, western blotting, and oxycodone treatment.

Main Results:

  • 38 DEGs identified between normal and vincristine-treated rats.
  • Enriched pathways include cAMP metabolism, inflammatory response, cell proliferation regulation, and chemokine signaling.
  • Vincristine induced dose-dependent inflammation in microglia, upregulating CXCL10, CXCL9, SFRP2, PF4, and downregulating IRF7 mRNA.
  • Oxycodone reduced CXCL10 and CXCL9 protein expression in activated microglia.

Conclusions:

  • Bioinformatics analysis revealed key DEGs and pathways in vincristine-induced neuropathic pain.
  • Oxycodone demonstrates potential to alleviate vincristine-induced inflammatory signaling in microglia by downregulating specific DEGs.
  • Further research is warranted to elucidate the precise molecular mechanisms.

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