Nucleotide Analysis in Korean Dairy Products Using High- Performance Liquid Chromatography with Diode Array Detector

Area of Science:

• Analytical Chemistry
• Food Science
• Biochemistry

Background:

• Nucleotides are vital for cellular processes and are added to infant formulas and dairy products.
• Existing domestic analytical methods for nucleotide content are insufficient, necessitating new approaches.

Purpose of the Study:

• To develop and validate a rapid, simple analytical method for quantifying five key nucleotides in dairy products.
• To improve upon existing methods by optimizing extraction and chromatographic conditions for speed and efficiency.

Main Methods:

• Solid phase extraction (SPE) for sample clean-up.
• High-performance liquid chromatography with a diode array detector (HPLC-DAD).
• Optimized extraction solvent (EDTA-Na) and isocratic elution for faster analysis.

Main Results:

• The improved method reduced analysis time from 40 to 25 minutes.
• Method validation demonstrated high accuracy (84.69%-102.72%) and precision (1.51%-6.82%).
• The method successfully quantified nucleotides in infant formulas (1.99-29.39 mg/100 g) and cow milk (0.28-0.83 mg/100 g).

Conclusions:

• The enhanced HPLC-DAD method provides a rapid and reliable means for nucleotide analysis in dairy products.
• This improved method is suitable for routine quality control of infant formulas and other dairy items.
• The study addresses the need for efficient domestic analytical techniques for nucleotide determination.