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Human T lymphocyte cell line producing colony-stimulating activity.

D W Golde, S G Quan, M J Cline

    Blood
    |November 1, 1978
    PubMed
    Summary

    Researchers developed a human T lymphocyte cell line that produces potent colony-stimulating activity (CSA). This cell line offers a consistent source for studying CSA and its role in regulating granulocyte and monocyte stem cells.

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    Area of Science:

    • Immunology
    • Hematology
    • Cell Biology

    Background:

    • Hairy-cell leukemia is a rare lymphoid malignancy.
    • T lymphocytes play a role in regulating hematopoiesis.
    • Colony-stimulating activity (CSA) is crucial for blood cell production.

    Purpose of the Study:

    • To establish a permanent human T lymphocyte cell line.
    • To characterize the colony-stimulating activity (CSA) produced by this cell line.
    • To investigate the potential of this cell line as a source for CSA.

    Main Methods:

    • Derivation of a T lymphocyte cell line from a patient with T-cell hairy-cell leukemia.
    • Culture of the cell line and collection of supernatant.
    • Assay of CSA in supernatant for stimulating human bone marrow colony formation.
    • Characterization of cell line properties (rosetting, PHA response, immunofluorescence).

    Main Results:

    • A permanent human T lymphocyte cell line was established.
    • The cell line regularly produced potent CSA in culture supernatant.
    • CSA production was augmented by phytohemagglutinin (PHA).
    • The supernatant stimulated colony formation from human bone marrow cells.

    Conclusions:

    • The established T lymphocyte line provides a valuable and consistent source of human CSA.
    • This cell line facilitates further research into CSA production and its regulatory mechanisms.
    • It enables detailed studies on T lymphocyte interactions with granulocyte and monocyte stem cells.

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