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Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion
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Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion

Published on: March 1, 2019

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Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion

Cheng-Yu Chen1, Ray-Jade Chen2, Gilbert Aaron Lee3

  • 1Research Center of Translational Imaging, College of Medicine, Taipei Medical University; Radiogenomic Research Center, Taipei Medical University Hospital, Taipei Medical University; Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University; Department of Medical Imaging, Taipei Medical University Hospital, Taipei Medical University; Department of Medical Research, Taipei Medical University Hospital, Taipei Medical University.

Journal of Visualized Experiments : Jove
|March 19, 2019
PubMed

Insights

This study introduces a reliable two-vessel occlusion mouse model for cerebral ischemia-reperfusion. The model demonstrates stable infarct size and aids in studying immune response and neuronal recovery post-stroke.

Area of Science:

  • Neuroscience
  • Immunology
  • Stroke Research

Background:

  • Cerebral ischemia-reperfusion is a critical condition leading to stroke.
  • Existing mouse models for studying stroke have limitations due to anatomical variations affecting infarct size.
  • A reproducible model is needed to investigate stroke pathophysiology and test therapies.

Purpose of the Study:

  • To establish and validate a reproducible two-vessel occlusion mouse model for cerebral ischemia-reperfusion.
  • To analyze infarct size stability and behavioral deficits in this model.
  • To investigate immune cell infiltration and neuronal loss during reperfusion.

Main Methods:

  • A two-vessel occlusion model was created by distally ligating the right middle cerebral artery (MCA) and right common carotid artery (CCA) in mice.
  • Blood flow was interrupted for a defined period, followed by reperfusion.
  • Infarct volume, behavioral deficits, immune cell infiltration, and neuronal loss were assessed.

Main Results:

  • The two-vessel occlusion model produced a stable infarct size and induced behavioral deficits.
  • Peripheral immune cells infiltrated the ischemic brain within 24 hours.
  • Longer reperfusion durations were associated with reduced neuronal loss in the cortical area.

Conclusions:

  • The established two-vessel occlusion model offers a reliable method for studying cerebral ischemia-reperfusion.
  • This model is suitable for investigating the role of the immune response in stroke.
  • The findings highlight the potential for neuronal recovery during reperfusion, influenced by duration.

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