Erratum: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

An erratum was issued for: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes.  There were typos in the Protocol section and Table 2. Step 3.1.1 in the Protocol was updated from: Add 80 µl of 1 mM Pefa Bloc and 60 mg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling). to: Add 80 µl of 1 mM Pefa Bloc and 60 µg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling). Step 5.2 in the Protocol was updated from: Further dilute the Control Peptide from step 5.1 (20 mM) 1:100 into the MHC II Master Mix from step 3.2. to: Further dilute the Control Peptide from step 5.1 (20 µM) 1:100 into the MHC II Master Mix from step 3.2. Table 2 was updated from: MHC II Allele DRB1*: 1501 MHC II Stock Concentration (mg/ml) 1.3 MHC II Stock Concentration (mM) 20 Vol. MHC II Stock to Add (ml) 14.65 Vol. Reaction Buffer to Add (ml): 2885.35 MHC II Master Mix Concentration (nM) 101 to: MHC II Allele DRB1*: 1501 MHC II Stock Concentration (mg/ml) 1.3 MHC II Stock Concentration (μM) 20 Vol. MHC II Stock to Add (μl) 14.65 Vol. Reaction Buffer to Add (μl): 2885.35 MHC II Master Mix Concentration (nM) 101.