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Updated: Jan 27, 2026

A Micropatterning Assay for Measuring Cell Chirality
Published on: March 11, 2022
Large-Ring Cyclodextrins as Chiral Selectors for Enantiomeric Pharmaceuticals
Christian Sonnendecker1, Sebastian Thürmann2, Cédric Przybylski3
1Dept. of Microbiology and Bioprocess Technology, Institute of Biochemistry, Leipzig University, Johannisallee 23, 04103, Leipzig, Germany.
Abstract:
Large-ring cyclodextrins (CD) are cyclic glucans composed of 9 or more α-1,4-linked glucose units. They are minor side products of bacterial glucanotransferases (CGTases, EC 2.4.1.19) and have previously been available only in very small amounts for studies of their properties in supramolecular complex formation reactions. We engineered a CGTase to synthesize mainly large-ring CD facilitating their preparation in larger amounts. By reversed phase chromatography, we obtained single CD samples composed of 10 to 12 glucose units (CD10, CD11, and CD12) with a purity of >90 %. Their identity was confirmed by high resolution mass spectrometry and fragmentation analysis. We demonstrated the non-toxicity of CD10-CD12 for human cell lines by a cell proliferation assay and impedimetric monitoring. We then showed that CD10 and CD11 are efficient chiral selectors for the capillary electrophoretic separation of the enantiomeric pharmaceuticals fluvastatin, mefloquine, carvedilol, and primaquine.
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