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Updated: Jan 27, 2026
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Experimental Models to Study the Neuroprotection of Acidic Postconditioning Against Cerebral Ischemia
Published on: July 31, 2017
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Ischemic Postconditioning Recovers Cortex Ascorbic Acid during Ischemia/Reperfusion Monitored with an Online
Dalei Wang1, Xianchan Li1, Ying Jiang1
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems , Institute of Chemistry, the Chinese Academy of Sciences (CAS), CAS Research/Education Center for Excellence in Molecule Science , Beijing 100190 , China.
ACS Chemical Neuroscience
|March 19, 2019
Summary
Ischemic postconditioning protects the brain after stroke. An online electrochemical system (OECS) effectively monitors cortex ascorbic acid levels, evaluating postconditioning
Area of Science:
- Neuroscience
- Biochemistry
- Medical Technology
Background:
- Ischemic postconditioning shows promise for neuroprotection after stroke.
- Evaluating its effectiveness during the acute phase requires reliable methods.
- Current methods lack real-time assessment of neuroprotective efficiency.
Purpose of the Study:
- To investigate cortex ascorbic acid dynamics during cerebral ischemia and reperfusion.
- To evaluate the neuroprotective efficiency of ischemic postconditioning using an online electrochemical system (OECS).
- To establish OECS as a platform for assessing postconditioning efficacy in acute ischemic stroke.
Main Methods:
- Utilized an online electrochemical system (OECS) to monitor cortex ascorbic acid.
- Induced cerebral ischemia/reperfusion in a model.
- Assessed injury and neuronal function via triphenyltetrazolium chloride staining, immunohistochemistry, and electrophysiology.
Main Results:
- Cortex ascorbic acid sharply increased post-ischemia and further rose upon reperfusion without postconditioning.
- Ischemic postconditioning caused cortex ascorbic acid to drop and recover to baseline.
- Ascorbic acid recovery correlated with improved neural function and reduced oxidative damage.
Conclusions:
- OECS monitoring of cortex ascorbic acid effectively evaluates ischemic postconditioning's neuroprotective efficiency.
- This method aids in screening optimal postconditioning parameters for ischemic stroke.
- OECS provides a valuable tool for acute phase assessment of neuroprotection.
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