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On-Chip Endothelial Inflammatory Phenotyping
Published on: July 21, 2012
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Differential inflammatory landscape stimulus during titanium surfaces obtained osteogenic phenotype
Georgia da S Feltran1, Fábio Bezerra1, Célio Júnior da Costa Fernandes1
1Department of Chemistry and Biochemistry, Bioscience Institute, State University of São Paulo - UNESP, campus Botucatu, Botucatu, São Paulo, Brazil.
Journal of Biomedical Materials Research. Part A
|March 19, 2019
Summary
The nanoscaled hydroxyapatite-blasted titanium surface (nHA) promotes an anti-inflammatory response by modulating Sonic Hedgehog (Shh) signaling, influencing inflammatory gene expression and potentially impacting osteoclastogenesis around implants.
Area of Science:
- Biomaterials Science
- Immunology
- Cell Biology
Background:
- Understanding the molecular mechanisms of inflammatory responses to titanium (Ti) nanotextured surfaces is crucial for dental implantology.
- Different Ti surface topographies, including machined (Mac), double acid-etched (DAE), and nanoscaled hydroxyapatite-blasted (nHA), elicit varied cellular responses.
Purpose of the Study:
- To investigate the inflammatory gene expression and signaling pathways activated by different Ti nanotextured surfaces.
- To elucidate the role of Sonic Hedgehog (Shh) signaling in modulating the inflammatory response to nHA surfaces.
Main Methods:
- Pre-osteoblast cells were cultured directly on or indirectly exposed to conditioned media from Mac, DAE, and nHA Ti surfaces for up to 10 days.
- Quantitative gene expression analysis was performed for inflammatory cytokines (IL1ß, IL6, IL10, IL33, IL18), inflammasome components (NLRP3, ASC1, CASP1), and Shh signaling pathway genes (Shh, Smo, Ptc).
- Bioinformatic interactome analysis was used to identify signaling pathways and mediators.
Main Results:
- The nHA surface induced dynamic expression of inflammatory genes, including IL1ß, IL6, IL10, IL33 (direct) and IL6, IL10, IL18 (indirect).
- Inflammasome components (NLRP3, ASC1, CASP1) were upregulated, indicating inflammasome involvement.
- Sonic Hedgehog (Shh) signaling pathway genes (Shh, Smo) were upregulated, while Patched (Ptc) was downregulated in response to nHA, correlating with increased IL10 expression, modulated by Src kinase.
Conclusions:
- The nHA surface promotes an anti-inflammatory profile, characterized by increased IL10 expression, mediated by the Sonic Hedgehog (Shh) signaling pathway.
- This Shh-driven anti-inflammatory response may contribute to reduced RANKL expression, potentially limiting osteoclastogenesis around Ti implants.
- The findings highlight the importance of nanotexturing in modulating cellular inflammatory responses for improved biomaterial integration.
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