Related Experiment Video
Updated: Jan 27, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
A nano sensor for sensitive and selective detection of Cu2+ based on fluorescein: Cell imaging and drinking water
Prasad G Mahajan1, Nilam C Dige2, Balasaheb D Vanjare1
1Department of Chemistry, Kongju National University, Gongju, Chungnam 32588, Republic of Korea.
Abstract:
A fluorescein-based nano probe was designed and synthesized for ultra-sensitive detection of Cu2+ in aqueous solution. The formation of fluorescent organic nanoparticles confirmed by using particle size analysis and scanning electron microscopy. UV-Vis. absorption and fluorescence spectroscopy displays excellent photophysical properties of prepared nanoparticles as compared to parent molecule i.e. N-(3',6'-dihydroxy-3-oxo-3,3a-dihydrospiro[isoindole-1,9'-xanthene]-2(7aH)-yl)-1-naphthamide (FNH) in acetone. A series of 18 metal ion was examined with FNH nanoparticles (FNHNPs) to examine the change in fluorescence response. Pleasingly, only copper ion (Cu2+) shows selective and sensitive fluorescence enhancement effect, which discussed on chelation-enhanced fluorescence phenomenon. Other competing metal ions does not affect the FNHNPs fluorescence enhancement induced by Cu2+ ion. The excited state complexation through chelation-enhanced fluorescence of FNHNPs was further supported by UV-Vis. absorption and fluorescence decay titration of FNHNPs with and without the addition of Cu2+. The present investigation approach serves extremely low detection limit of 1.62 ng/mL (0.024 μM) for Cu2+ in aqueous solution. In addition, benefit of present study includes practical application for the quantitative estimation of Cu2+ in drinking water sample and intracellular cell imaging for Cu2+.
Related Concept Videos
Water: A Bronsted-Lowry Acid and Base
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
States of Water
Water freezes when the intermolecular forces are greater than the kinetic energy. Unlike most other substances, water is less dense in its solid state than in its liquid state. This is because each water molecule can form...
Water and Mineral Acquisition
What is Natural Selection?
Quality of Water

